Abstract
Glaucoma occurs when there are imbalances between the production and the drainage of the eye liquid. The vast majority of the aqueous humor leaves the eye through the trabecular meshwork (TM). The cause of hypertonicity may be due to an alteration in the thickness of the TM. In the majority of cases the molecular changes that determine primary openangle glaucoma (POAG) are unclear. However, it has been hypothesized that the significant increase in the extracellular matrix (ECM) of the fibrillary bands in the TM is associated with possible inflammatory conditions. In this study the tissue distribution of interleukin (IL)6, IL1β, transforming growth factor-β1 (TGFβ1), vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNFα) was analyzed in TM samples from patients with POAG by immunohistochemistry. Seven specimens from patients with POAG and three control tissues were analyzed by immunohistochemistry using specific antibodies against these cytokines. Morphological changes in the TM, such as increased cell content, macrophages, fibrosis and accumulation of neutrophils, were observed by transmission electron microscopy. In human TM tissues, an evident immunoreactivity for IL6, IL1β and TNFα was observed in patients with POAG when compared with the control subjects, indicating that these cytokines may be correlated with disease activity. TM endothelial cells secrete a number of factors and cytokines that modulate the functions of the cells and the ECM of the conventional outflow pathway. In the TM in glaucoma, macrophages produce cytokines, including IL6, IL1β and TNFα, leading to an acute inflammatory response and recruitment of other immune cells, including T lymphocytes. In addition, TGFβ1 regulates and induces the expression of IL6 in TM that indirectly induces angiogenesis by stimulating VEGF expression. The present results support previous evidence that suggests that growth factors and cytokines can induce ECM remodelling and alter cytoskeletal interactions in the TM.
| Original language | English |
|---|---|
| Pages (from-to) | 1384-1390 |
| Number of pages | 7 |
| Journal | Molecular Medicine Reports |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2015 |
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Keywords
- Immunohistochemistry
- Interleukin1β
- Interleukin6
- Intraocular pressure
- Primary openangle glaucoma
- Tumor growth factor β1
- Tumor necrosis factor α
- Vascular endothelial growth factor
ASJC Scopus subject areas
- Biochemistry
- Cancer Research
- Genetics
- Molecular Biology
- Molecular Medicine
- Oncology
- Medicine(all)
Cite this
Potential regulatory molecules in the human trabecular meshwork of patients with glaucoma : Immunohistochemical profile of a number of inflammatory cytokines. / Taurone, Samanta; Ripandelli, Guido; Pacella, Elena; Bianchi, Enrica; Plateroti, Andrea Maria; De Vito, Stefania; Plateroti, Pasquale; Grippaudo, Francesca Romana; Cavallotti, Carlo; Artico, Marco.
In: Molecular Medicine Reports, Vol. 11, No. 2, 01.02.2015, p. 1384-1390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potential regulatory molecules in the human trabecular meshwork of patients with glaucoma
T2 - Immunohistochemical profile of a number of inflammatory cytokines
AU - Taurone, Samanta
AU - Ripandelli, Guido
AU - Pacella, Elena
AU - Bianchi, Enrica
AU - Plateroti, Andrea Maria
AU - De Vito, Stefania
AU - Plateroti, Pasquale
AU - Grippaudo, Francesca Romana
AU - Cavallotti, Carlo
AU - Artico, Marco
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Glaucoma occurs when there are imbalances between the production and the drainage of the eye liquid. The vast majority of the aqueous humor leaves the eye through the trabecular meshwork (TM). The cause of hypertonicity may be due to an alteration in the thickness of the TM. In the majority of cases the molecular changes that determine primary openangle glaucoma (POAG) are unclear. However, it has been hypothesized that the significant increase in the extracellular matrix (ECM) of the fibrillary bands in the TM is associated with possible inflammatory conditions. In this study the tissue distribution of interleukin (IL)6, IL1β, transforming growth factor-β1 (TGFβ1), vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNFα) was analyzed in TM samples from patients with POAG by immunohistochemistry. Seven specimens from patients with POAG and three control tissues were analyzed by immunohistochemistry using specific antibodies against these cytokines. Morphological changes in the TM, such as increased cell content, macrophages, fibrosis and accumulation of neutrophils, were observed by transmission electron microscopy. In human TM tissues, an evident immunoreactivity for IL6, IL1β and TNFα was observed in patients with POAG when compared with the control subjects, indicating that these cytokines may be correlated with disease activity. TM endothelial cells secrete a number of factors and cytokines that modulate the functions of the cells and the ECM of the conventional outflow pathway. In the TM in glaucoma, macrophages produce cytokines, including IL6, IL1β and TNFα, leading to an acute inflammatory response and recruitment of other immune cells, including T lymphocytes. In addition, TGFβ1 regulates and induces the expression of IL6 in TM that indirectly induces angiogenesis by stimulating VEGF expression. The present results support previous evidence that suggests that growth factors and cytokines can induce ECM remodelling and alter cytoskeletal interactions in the TM.
AB - Glaucoma occurs when there are imbalances between the production and the drainage of the eye liquid. The vast majority of the aqueous humor leaves the eye through the trabecular meshwork (TM). The cause of hypertonicity may be due to an alteration in the thickness of the TM. In the majority of cases the molecular changes that determine primary openangle glaucoma (POAG) are unclear. However, it has been hypothesized that the significant increase in the extracellular matrix (ECM) of the fibrillary bands in the TM is associated with possible inflammatory conditions. In this study the tissue distribution of interleukin (IL)6, IL1β, transforming growth factor-β1 (TGFβ1), vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNFα) was analyzed in TM samples from patients with POAG by immunohistochemistry. Seven specimens from patients with POAG and three control tissues were analyzed by immunohistochemistry using specific antibodies against these cytokines. Morphological changes in the TM, such as increased cell content, macrophages, fibrosis and accumulation of neutrophils, were observed by transmission electron microscopy. In human TM tissues, an evident immunoreactivity for IL6, IL1β and TNFα was observed in patients with POAG when compared with the control subjects, indicating that these cytokines may be correlated with disease activity. TM endothelial cells secrete a number of factors and cytokines that modulate the functions of the cells and the ECM of the conventional outflow pathway. In the TM in glaucoma, macrophages produce cytokines, including IL6, IL1β and TNFα, leading to an acute inflammatory response and recruitment of other immune cells, including T lymphocytes. In addition, TGFβ1 regulates and induces the expression of IL6 in TM that indirectly induces angiogenesis by stimulating VEGF expression. The present results support previous evidence that suggests that growth factors and cytokines can induce ECM remodelling and alter cytoskeletal interactions in the TM.
KW - Immunohistochemistry
KW - Interleukin1β
KW - Interleukin6
KW - Intraocular pressure
KW - Primary openangle glaucoma
KW - Tumor growth factor β1
KW - Tumor necrosis factor α
KW - Vascular endothelial growth factor
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U2 - 10.3892/mmr.2014.2772
DO - 10.3892/mmr.2014.2772
M3 - Article
VL - 11
SP - 1384
EP - 1390
JO - Molecular Medicine Reports
JF - Molecular Medicine Reports
SN - 1791-2997
IS - 2
ER -