TY - JOUR
T1 - Potential role of circulating microRNAs as early markers of preeclampsia
AU - Ura, Blendi
AU - Feriotto, Giordana
AU - Monasta, Lorenzo
AU - Bilel, Sabrine
AU - Zweyer, Marina
AU - Celeghini, Claudio
PY - 2014
Y1 - 2014
N2 - Objective: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n=24) compared to women with normal pregnancy (n=24). Materials and Methods: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. Results: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. Conclusion: Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
AB - Objective: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n=24) compared to women with normal pregnancy (n=24). Materials and Methods: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. Results: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. Conclusion: Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
KW - Early biomarkers
KW - MicroRNA
KW - Preeclampsia
UR - http://www.scopus.com/inward/record.url?scp=84903942158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903942158&partnerID=8YFLogxK
U2 - 10.1016/j.tjog.2014.03.001
DO - 10.1016/j.tjog.2014.03.001
M3 - Article
C2 - 25017274
AN - SCOPUS:84903942158
VL - 53
SP - 232
EP - 234
JO - Taiwanese Journal of Obstetrics and Gynecology
JF - Taiwanese Journal of Obstetrics and Gynecology
SN - 1028-4559
IS - 2
ER -