Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines

Patrizia Russo, Silvio Parodi, Giovanna Billi, Cristina Oliva, Marco Venturini, Elvira Noviello, PierFranco Conte

Research output: Contribution to journalArticlepeer-review

Abstract

The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PA1), while 3 cell lines (IGROV1, SKOV3, Me180) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone.

Original languageEnglish
Pages (from-to)684-687
Number of pages4
JournalJapanese Journal of Cancer Research
Volume83
Issue number7
Publication statusPublished - Jul 1992

Keywords

  • Mitoxantrone cytotoxicity
  • Ovarian cancer cell
  • TNF

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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