Potentiation of Chemotherapy in Companion Animals with Spontaneous Large Neoplasms by Application of Biphasic Electric Pulses

Enrico P. Spugnini, A. Porrello

Research output: Contribution to journalArticle

Abstract

The objectives of this phase I/II study were: i) to determine whether electrochemotherapy (intralesional bleomycin + electric pulses) could be effective in companion animals with different, large neoplasms compared to chemotherapy (conventional intralesional bleomycin); ii) to identify potential toxicities; iii) to preliminarily assess the electric field requirements. Twenty-two patients received intralesional bleomycin + administration of permeabilizing electric pulses. Specifically, after the injection of the drug, sequences of 8 biphasic electric pulses lasting 50 + 50 μs each, with 1 ms interpulse intervals, were delivered in bursts of 1300 V/cm for cutaneous and soft tissue lesions, and of 800 V/cm for oral mucosal and exposed soft tissue neoplasms, using caliper electrodes. The treatment was well tolerated and side effects were infrequent. Nevertheless, two previously unreported toxicities (drug-induced vasculitis and pulmonary thromboembolism) have been identified. A high response rate (complete remission + partial remission: > 80%), often long lasting (> 40%) was obtained. Furthermore, results of this trial were compared to a subset of veterinary cancer patients treated with bleomycin single agent, observing a remarkable superiority of the combined treatment (p <0.01). Altogether, results suggest that electrochemotherapy is a potentially advantageous rescue protocol for bulky, even relapsing neoplasms of companion animals. Further investigations in this field might allow developing improved protocols for the treatment of down-staged relapsing cancer in pets as well as in humans.

Original languageEnglish
Pages (from-to)571-580
Number of pages10
JournalJournal of Experimental and Clinical Cancer Research
Volume22
Issue number4
Publication statusPublished - Dec 2003

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Keywords

  • Animal models
  • Bleomycin
  • Electric pulses
  • Electrochemotherapy
  • Neoplasms
  • Pets

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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