PPARgamma Promoter Polymorphisms and Acute Coronary Syndrome

L. Evangelisti, M. Attanasio, L. Lucarini, F. Sofi, R. Marcucci, C. Giglioli, S. Valente, GF Gensini, R. Abbate, G. Pepe

Research output: Contribution to journalArticle

Abstract

Background: PPARgamma (PPARg) is a nuclear transcription factor involved in the control of lipid and glucose homeostasis. Two PPARg common polymorphisms, Pro12Ala and 161C > T, have been found to be associated with cardiovascular disease. In this study, in addition to PPARg coding region, we looked for genetic variations in promoters and their association with acute coronary syndrome (ACS). Methods: We studied 202 Italian patients with ACS, and 295 healthy Italian subjects by dHPLC (denaturing high-performance liquid chromatography), heteroduplex analysis and direct sequencing or RFLP (restriction fragment length polymorphism) analysis for screening mutations. Results: We identified 7 new and 2 already published polymorphisms in PPARg promoters. The C > T93695 (promoter 4) mutation showed significantly different genotype distribution and allele frequency between controls and ACS patients (p <0.001); the T allele conferred a protection against ACS at both univariate (OR: 0.45, 95% CI 0.29-0.69: p <0.001) and multivariate analysis adjusted for sex, age and traditional cardiovascular risk factors (OR: 0.44, 95% CI 0.25-0.76: p <0.005). Moreover, the 161C > T polymorphism allele frequency (p = 0.03) and genotype distribution (p = 0.015) resulted to be different in ACS group if compared to healthy controls. Conclusions: The protective role of 93695C > T polymorphism in PPARg promoter in ACS suggests that PPARg genetic variants may affect the susceptibility to atherosclerotic diseases.

Original languageEnglish
Pages (from-to)186-191
Number of pages6
JournalAtherosclerosis
Volume205
Issue number1
DOIs
Publication statusPublished - Jul 2009

Keywords

  • Acute coronary syndrome
  • Genetics
  • Metabolism
  • Polymorphism
  • PPARgamma
  • Transcription factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'PPARgamma Promoter Polymorphisms and Acute Coronary Syndrome'. Together they form a unique fingerprint.

  • Cite this

    Evangelisti, L., Attanasio, M., Lucarini, L., Sofi, F., Marcucci, R., Giglioli, C., Valente, S., Gensini, GF., Abbate, R., & Pepe, G. (2009). PPARgamma Promoter Polymorphisms and Acute Coronary Syndrome. Atherosclerosis, 205(1), 186-191. https://doi.org/10.1016/j.atherosclerosis.2008.11.009