Abstract
The protein product of the retinoblastoma (RB) gene is necessary for the completion of the muscle differentiation program and for myogenic basic helix-loop-helix-dependent transcription. In fact, in addition to induction and maintenance of permanent cell cycle withdrawal through negative regulation of E2F-responsive genes involved in proliferation, pRb also plays a positive role in the activation of muscle-specific genes. In pRb-/- myocytes, the expression of late myogenic markers is defective and myoblast fusion into myotubes occurs without irreversible cell cycle exit. This evidence demonstrates only a partial functional redundancy between pRb and its relatives p107 and pRb2/p130, as these pRb-/- multinucleated cells, which display p107 levels higher than normal myotubes, respond to mitogens with cell cycle re-entry and DNA synthesis. At the molecular level, pRb myogenic functions are mediated by cooperation with MyoD, Myocyte enhancer factor 2 (MEF2), High mobility group box protein-1 (HBP1) and histone deacetylase1, affecting chromatin configuration and tissue-specific transcription, and by post-translational modification in response to intracellular signaling cascades.
| Original language | English |
|---|---|
| Pages (from-to) | 5244-5249 |
| Number of pages | 6 |
| Journal | Oncogene |
| Volume | 25 |
| Issue number | 38 |
| DOIs | |
| Publication status | Published - Aug 28 2006 |
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Keywords
- Cell cycle withdrawal
- Differentiation
- MyoD
- Myogenesis
- Retinoblastoma gene
- Tissue-specific transcription
ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics
Cite this
pRb : Master of differentiation. Coupling irreversible cell cycle withdrawal with induction of muscle-specific transcription. / De Falco, G.; Comes, F.; Simone, C.
In: Oncogene, Vol. 25, No. 38, 28.08.2006, p. 5244-5249.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - pRb
T2 - Master of differentiation. Coupling irreversible cell cycle withdrawal with induction of muscle-specific transcription
AU - De Falco, G.
AU - Comes, F.
AU - Simone, C.
PY - 2006/8/28
Y1 - 2006/8/28
N2 - The protein product of the retinoblastoma (RB) gene is necessary for the completion of the muscle differentiation program and for myogenic basic helix-loop-helix-dependent transcription. In fact, in addition to induction and maintenance of permanent cell cycle withdrawal through negative regulation of E2F-responsive genes involved in proliferation, pRb also plays a positive role in the activation of muscle-specific genes. In pRb-/- myocytes, the expression of late myogenic markers is defective and myoblast fusion into myotubes occurs without irreversible cell cycle exit. This evidence demonstrates only a partial functional redundancy between pRb and its relatives p107 and pRb2/p130, as these pRb-/- multinucleated cells, which display p107 levels higher than normal myotubes, respond to mitogens with cell cycle re-entry and DNA synthesis. At the molecular level, pRb myogenic functions are mediated by cooperation with MyoD, Myocyte enhancer factor 2 (MEF2), High mobility group box protein-1 (HBP1) and histone deacetylase1, affecting chromatin configuration and tissue-specific transcription, and by post-translational modification in response to intracellular signaling cascades.
AB - The protein product of the retinoblastoma (RB) gene is necessary for the completion of the muscle differentiation program and for myogenic basic helix-loop-helix-dependent transcription. In fact, in addition to induction and maintenance of permanent cell cycle withdrawal through negative regulation of E2F-responsive genes involved in proliferation, pRb also plays a positive role in the activation of muscle-specific genes. In pRb-/- myocytes, the expression of late myogenic markers is defective and myoblast fusion into myotubes occurs without irreversible cell cycle exit. This evidence demonstrates only a partial functional redundancy between pRb and its relatives p107 and pRb2/p130, as these pRb-/- multinucleated cells, which display p107 levels higher than normal myotubes, respond to mitogens with cell cycle re-entry and DNA synthesis. At the molecular level, pRb myogenic functions are mediated by cooperation with MyoD, Myocyte enhancer factor 2 (MEF2), High mobility group box protein-1 (HBP1) and histone deacetylase1, affecting chromatin configuration and tissue-specific transcription, and by post-translational modification in response to intracellular signaling cascades.
KW - Cell cycle withdrawal
KW - Differentiation
KW - MyoD
KW - Myogenesis
KW - Retinoblastoma gene
KW - Tissue-specific transcription
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UR - http://www.scopus.com/inward/citedby.url?scp=33748035693&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1209623
DO - 10.1038/sj.onc.1209623
M3 - Article
C2 - 16936743
AN - SCOPUS:33748035693
VL - 25
SP - 5244
EP - 5249
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 38
ER -