Abstract
It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the nucleus and that cell cycle-dependent binding with E2F4 changes also as a function of their subnuclear localization. Namely, in the nucleoplasm pRb2/p130-E2F4 complexes are more numerous during G0/G1 while in the nucleolus they increase in S phase. Partially different functions for p107 are suggested since p107-E2F4 complexes in the nucleoplasm are more numerous is S phase with respect to G0/G1 and no cell cycle change is observed in the nucleolus. In a second series of experiments we showed that pRb2/ p130, p107, E2F4, and pRb2/p130-HDAC1 complexes are all inner nuclear matrix-associated proteins and localize to sites different from pRb/p105 ones. We provide further evidence of multiple and partially distinct retinoblastoma protein family functional roles during cell cycle. Moreover, our data support emerging evidence for functional interrelationships between nuclear structure and gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 34-44 |
| Number of pages | 11 |
| Journal | Journal of Cellular Physiology |
| Volume | 189 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
pRb2/p130 and p107 control cell growth by multiple strategies and in association with different compartments within the nucleus. / Zini, Nicoletta; Trimarchi, Carmela; Claudio, Pier Paolo; Stiegler, Peter; Marinelli, Fiorenzo; Maltarello, Maria Cristina; La Sala, Dario; De Falco, Giulia; Russo, Giuseppe; Ammirati, Giuseppe; Maraldi, Nadir Mario; Giordano, Antonio; Cinti, Caterina.
In: Journal of Cellular Physiology, Vol. 189, No. 1, 2001, p. 34-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - pRb2/p130 and p107 control cell growth by multiple strategies and in association with different compartments within the nucleus
AU - Zini, Nicoletta
AU - Trimarchi, Carmela
AU - Claudio, Pier Paolo
AU - Stiegler, Peter
AU - Marinelli, Fiorenzo
AU - Maltarello, Maria Cristina
AU - La Sala, Dario
AU - De Falco, Giulia
AU - Russo, Giuseppe
AU - Ammirati, Giuseppe
AU - Maraldi, Nadir Mario
AU - Giordano, Antonio
AU - Cinti, Caterina
PY - 2001
Y1 - 2001
N2 - It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the nucleus and that cell cycle-dependent binding with E2F4 changes also as a function of their subnuclear localization. Namely, in the nucleoplasm pRb2/p130-E2F4 complexes are more numerous during G0/G1 while in the nucleolus they increase in S phase. Partially different functions for p107 are suggested since p107-E2F4 complexes in the nucleoplasm are more numerous is S phase with respect to G0/G1 and no cell cycle change is observed in the nucleolus. In a second series of experiments we showed that pRb2/ p130, p107, E2F4, and pRb2/p130-HDAC1 complexes are all inner nuclear matrix-associated proteins and localize to sites different from pRb/p105 ones. We provide further evidence of multiple and partially distinct retinoblastoma protein family functional roles during cell cycle. Moreover, our data support emerging evidence for functional interrelationships between nuclear structure and gene expression.
AB - It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the nucleus and that cell cycle-dependent binding with E2F4 changes also as a function of their subnuclear localization. Namely, in the nucleoplasm pRb2/p130-E2F4 complexes are more numerous during G0/G1 while in the nucleolus they increase in S phase. Partially different functions for p107 are suggested since p107-E2F4 complexes in the nucleoplasm are more numerous is S phase with respect to G0/G1 and no cell cycle change is observed in the nucleolus. In a second series of experiments we showed that pRb2/ p130, p107, E2F4, and pRb2/p130-HDAC1 complexes are all inner nuclear matrix-associated proteins and localize to sites different from pRb/p105 ones. We provide further evidence of multiple and partially distinct retinoblastoma protein family functional roles during cell cycle. Moreover, our data support emerging evidence for functional interrelationships between nuclear structure and gene expression.
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U2 - 10.1002/jcp.1135
DO - 10.1002/jcp.1135
M3 - Article
VL - 189
SP - 34
EP - 44
JO - Journal of cellular and comparative physiology
JF - Journal of cellular and comparative physiology
SN - 0021-9541
IS - 1
ER -