pRb2/p130 promotes radiation-induced cell death in the glioblastoma cell line HJC12 by p73 upregulation and Bcl-2 downregulation

Bruna Pucci, Pier Paolo Claudio, Valeria Masciullo, Lorenza Bellincampi, Alessandro Terrinoni, Kamel Khalili, Gerry Melino, Antonio Giordano

Research output: Contribution to journalArticlepeer-review

Abstract

This study shows that in the glioblastoma hamster cell line HJC12 the retinoblastoma family member pRb2/p130 enhances γ-radiation-induced cell death. In HJC12 cells the tetracycline-regulated expression of pRb2/p130 increased the percentage of γ-radiation-induced apoptotic cells from 27 to 47%. pRb2/p130 overexpression was associated with the downregulation of the anti-apoptotic factor Bcl-2 and the upregulation of the steady-state protein levels of the pro-apoptotic transcription factor p73. In particular, RT-PCR showed a significant increase in the expression of the p73δ isoform when pRb2/p130 was overexpressed. The ability of pRb2/p130 to modulate apoptosis was not associated with its role in mediating G0/G1 arrest during cell cycle progression. Our data suggest a role for pRb2/p130 in glioblastoma γ-radiation-induced cell death, indicating that the antitumoral action of pRb2/p130 can regulate both inhibition of cell cycle progression and induction of cell death.

Original languageEnglish
Pages (from-to)5897-5905
Number of pages9
JournalOncogene
Volume21
Issue number38
DOIs
Publication statusPublished - Aug 29 2002

Keywords

  • γ-radiation
  • Apoptosis
  • Bcl-2
  • Cell cycle
  • Glioblastoma
  • p73
  • pRb2/p130

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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