Pre-culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentials

M. G. Valorani, E. Montelatici, A. Germani, A. Biddle, D. D'Alessandro, R. Strollo, M. P. Patrizi, L. Lazzari, E. Nye, W. R. Otto, P. Pozzilli, M. R. Alison

Research output: Contribution to journalArticle

Abstract

Objectives: Hypoxia is an important factor in many aspects of stem-cell biology including their viability, proliferation, differentiation and migration. We evaluated whether low oxygen level (2%) affected human adipose tissue mesenchymal stem-cell (hAT-MSC) phenotype, population growth, viability, apoptosis, necrosis and their adipogenic and osteogenic differentiation potential. Materials and methods: hAT-MSCs from four human donors were cultured in growth medium under either normoxic or hypoxic conditions for 7 days and were then transferred to normoxic conditions to study their differentiation potential. Results: Hypoxia enhanced hAT-MSC expansion and viability, whereas expression of mesenchymal markers such as CD90, CD73 and endothelial progenitor cell marker CD34, remained unchanged. We also found that pre-culturing hAT-MSCs under hypoxia resulted in their enhanced ability to differentiate into adipocytes and osteocytes. Conclusions: This protocol could be useful for maximizing hAT-MSC potential to differentiate in vitro into the adipogenic and osteogenic lineages, for use in plastic and reconstructive surgery, and in tissue engineering strategies.

Original languageEnglish
Pages (from-to)225-238
Number of pages14
JournalCell Proliferation
Volume45
Issue number3
DOIs
Publication statusPublished - Jun 2012

ASJC Scopus subject areas

  • Cell Biology

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    Valorani, M. G., Montelatici, E., Germani, A., Biddle, A., D'Alessandro, D., Strollo, R., Patrizi, M. P., Lazzari, L., Nye, E., Otto, W. R., Pozzilli, P., & Alison, M. R. (2012). Pre-culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentials. Cell Proliferation, 45(3), 225-238. https://doi.org/10.1111/j.1365-2184.2012.00817.x