Pre-cut donor tissue for Descemet stripping automated keratoplasty: Anterior hinged lamella on versus off

Domenico Amato, Francesco Oddone, Mario Nubile, R. A M C Gisoldi, Carlo Maria Villani, Augusto Pocobelli

Research output: Contribution to journalArticle

Abstract

Aims: To compare two different techniques for preparation of pre-cut lamellar corneal tissue for Descemet stripping automated keratoplasty. Background/Methods: Eight donor lenticules were created with a full pass of the microkeratome blade, which resulted in a posterior lamella and a free cap (anterior lamella off group (AL-off)). Contralateral donor lenticules were created with an incomplete pass of the microkeratome blade, which resulted in a posterior lamella and a hinged anterior cap (anterior lamella on group (AL-on)). Endothelial cell density, cellular viability and corneal thickness were evaluated before dissection, and 4 and 24 h after dissection. Results: Average pre-cut endothelial cell density was 2552.25 (±105) and 2572.25 (±110) cells/mm2 in the AL-off group and AL-on group, respectively (p=0.7). At 24 h cell density was 2404.87 (±74) in the AL-off group (p

Original languageEnglish
Pages (from-to)519-522
Number of pages4
JournalBritish Journal of Ophthalmology
Volume94
Issue number4
DOIs
Publication statusPublished - Apr 2010

ASJC Scopus subject areas

  • Ophthalmology
  • Cellular and Molecular Neuroscience
  • Sensory Systems

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