Pre-lamin A processing is linked to heterochromatin organization

Giovanna Lattanzi, Maria Columbaro, Elisabetta Mattioli, Vittoria Cenni, Daria Camozzi, Manfred Wehnert, Spartaco Santi, Massimo Riccio, Rosalba Del Coco, Nadir M. Maraldi, Stefano Squarzoni, Roland Foisner, Cristina Capanni

Research output: Contribution to journalArticlepeer-review

Abstract

Pre-lamin A undergoes subsequent steps of post-translational modification at its C-terminus, including farnesylation, methylation, and cleavage by ZMPSTE24 metalloprotease. Here, we show that accumulation of different intermediates of pre-lamin A processing in nuclei, induced by expression of mutated pre-lamin A, differentially affected chromatin organization in human fibroblasts. Unprocessed (non-farnesylated) pre-lamin A accumulated in intranuclear foci, caused the redistribution of LAP2alpha and of the heterochromatin markers HP1 alpha and trimethyl-K9-histone 3, and triggered heterochromatin localization in the nuclear interior. In contrast, the farnesylated and carboxymethylated lamin A precursor accumulated at the nuclear periphery and caused loss of heterochromatin markers and Lap2alpha in enlarged nuclei. Interestingly, pre-lamin A bound both HP1alpha and LAP2alpha in vivo, but the farnesylated form showed reduced affinity for HP1alpha. Our data show a link between pre-lamin A processing and heterochromatin remodeling and have major implications for understanding molecular mechanisms of human diseases linked to mutations in lamins.

Original languageEnglish
Pages (from-to)1149-1159
Number of pages11
JournalJournal of Cellular Biochemistry
Volume102
Issue number5
DOIs
Publication statusPublished - Dec 1 2007

Keywords

  • Heterochromatin organization
  • HP1α, LAP2α
  • Lamin A
  • Pre-lamin A

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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