Pre-treatment of central venous catheters with the cathelicidin BMAP-28 enhances the efficacy of antistaphylococcal agents in the treatment of experimental catheter-related infection

Oscar Cirioni, Andrea Giacometti, Roberto Ghiselli, Cristina Bergnach, Fiorenza Orlando, Federico Mocchegiani, Carmela Silvestri, Alberto Licci, Barbara Skerlavaj, Margherita Zanetti, Vittorio Saba, Giorgio Scalise

Research output: Contribution to journalArticle

Abstract

An in vitro antibiotic susceptibility assay for Staphylococcus aureus biofilms developed on 96-well polystyrene tissue culture plates was performed to elucidate the activity of the 27 residues cathelicidin peptide BMAP-28, quinupristin/dalfopristin (Q/D), linezolid, and vancomycin. Efficacy studies were performed in a rat model of staphylococcal CVC infection. Silastic catheters were implanted into the superior cava. Twenty-four hours after implantation the catheters were filled with BMAP-28. Thirty minutes later rats were challenged via the CVC with 1.0 × 106 CFU of S. aureus strain Smith diffuse. Administration of antibiotics into the CVC at a concentration equal to the MBC observed using adherent cells, or at a much higher concentration (1024 μg/mL) began 24 h later. The inhibition activities of all antibiotics against adherent bacteria were at least two-four-fold lower that against freely growing cells. When antibiotics were used in BMAP-28 pre-treated wells, they showed higher activities. The in vivo studies showed that when CVCs were pre-treated with BMAP-28 or with a high dose of antibiotics, biofilm bacterial load was reduced from 107 to 103 CFU/mL and bacteremia reduced from 103 to 101 CFU/mL. When CVCs were treated with both BMAP-28 and antibiotics, biofilm bacterial load was further decreased to 101 CFU/mL and bacteremia was not detected. These results suggest that CVC pre-treated with BMAP-28 represents an attractive choice for the treatment of device-related infections caused by staphylococci.

Original languageEnglish
Pages (from-to)2104-2110
Number of pages7
JournalPeptides
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 2006

Keywords

  • Antibiotic-lock technique
  • Antimicrobial peptides
  • Biofilm
  • BMAP-28
  • CVC

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Pre-treatment of central venous catheters with the cathelicidin BMAP-28 enhances the efficacy of antistaphylococcal agents in the treatment of experimental catheter-related infection'. Together they form a unique fingerprint.

  • Cite this

    Cirioni, O., Giacometti, A., Ghiselli, R., Bergnach, C., Orlando, F., Mocchegiani, F., Silvestri, C., Licci, A., Skerlavaj, B., Zanetti, M., Saba, V., & Scalise, G. (2006). Pre-treatment of central venous catheters with the cathelicidin BMAP-28 enhances the efficacy of antistaphylococcal agents in the treatment of experimental catheter-related infection. Peptides, 27(9), 2104-2110. https://doi.org/10.1016/j.peptides.2006.03.007