Preclinical in vitro evaluation of hematotoxicity of the cisplatin-procaine complex DPR

Maurizio Viale, Silvia Minetti, Massimo Ottone, Roberto Lerza, Brunella Parodi, Ivo Pannacciulli

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated in vitro the inhibitory effect of cis-diaminechloro-[2-(diethylamino) ethyl 4-amino-benzoate, N4]-chlorideplatinum(II) monohydrochloride monohydrate (DPR) on colony formation by granulocyte/macrophage (CFU-GM) peripheral blood progenitor cells, representing a method to quantitate the toxicity of drugs to the hematopoietic system, and human leukemic cell lines. The results were compared with those obtained exposing cells to cisplatin and carboplatin. Our data showed that while DPR had a significantly better cytotoxic activity than cisplatin and carboplatin against HL60 and K562, and than carboplatin against Molt 4 cells, it showed 12 and 43 times less inhibitory effect on CFU-GM than cisplatin and carboplatin, respectively. These results suggest that the myelosuppressive activity of DPR could be lower than that of cisplatin and carboplatin, and, furthermore, that leukemic cells represent a preferential target for its cytotoxic activity compared to normal committed hemopoietic progenitor cells. All our results speak in favor of a better therapeutic index for DPR than for the other platinum compounds considered here.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalAnti-Cancer Drugs
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 2003

Keywords

  • CFU-GM
  • DPR
  • Hematotoxicity
  • In vitro

ASJC Scopus subject areas

  • Pharmacology
  • Cancer Research
  • Oncology

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