Preclinical pharmacology and safety of a novel avidin derivative for tissue-targeted delivery of radiolabelled biotin

Fiorella Petronzelli, Anna M. Anastasi, Angela Pelliccia, Daniela Santapaola, Claudio Albertoni, Antonio Rosi, Barbara Leoni, Liliana E. Ferrari, Giovanni Paganelli, Giancarlo Gramiccioli, Daniela Pesce, Anna M. Alfano, Maria A. Stasi, Rita De Santis

Research output: Contribution to journalArticlepeer-review

Abstract

We recently described an oxidized avidin variant, named AvidinOX ®, which is a product that chemically links to tissue proteins while maintaining the capacity to uptake intravenously administered biotin. Such product proved to be successful in targeting radionuclide therapy in a mouse model of inoperable breast cancer. Here, we show that the uptake of a single or multiple doses of biotin (up to five times), by the tissue-bound AvidinOX ®, is stable for 2weeks. Taking into account that oxidized avidin is the first chemically reactive protein to be proposed for clinical use, we evaluated its tolerability, immunogenicity and mutagenicity. Present in vitro data indicate that AvidinOX ® (up to 10μg/5×10 5 cells) does not affect cell viability or proliferation of PC3 human prostate cancer or 3T3 mouse fibroblast cell lines as well as primary mouse spleen cells. Safety pharmacology and toxicology studies were conducted using AvidinOX ® up to the highest concentration compatible with its solubility (about 12mg/mL), representing four times the product concentration intended for human use, and in the maximum administrable volume compatible with each study system. The intramuscular administration in rat and monkey induced a moderate to strong inflammatory response particularly after a second administration and consistently with the induction of an immune response. Interestingly, the intramuscular administration of AvidinOX ® to rodents and monkeys exhibiting very high anti-avidin antibody titres was well tolerated with no systemic symptoms of any kind. Intravenous administration of AvidinOX ®, performed to mimic an accidental injection of the dose intended for a local administration (15μL of 3.3mg/mL solution), showed significant localization of the product into the spleen not associated with uptake of the radiolabelled biotin intravenously injected after 24hr, thus suggesting rapid inactivation. No mutagenic activity was induced by oxidized avidin in prokaryotic and eukaryotic cells. Overall, the present data indicate that AvidinOX ® is well tolerated in rodents and non-human primates, thus supporting its clinical use within protocols of radionuclide therapy of inoperable tumour lesions.

Original languageEnglish
Pages (from-to)145-155
Number of pages11
JournalBasic and Clinical Pharmacology and Toxicology
Volume109
Issue number3
DOIs
Publication statusPublished - Sep 2011

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Fingerprint Dive into the research topics of 'Preclinical pharmacology and safety of a novel avidin derivative for tissue-targeted delivery of radiolabelled biotin'. Together they form a unique fingerprint.

Cite this