Abstract
Telomerase activity and telomerase reverse transcriptase (hTERT) expression are elevated in human malignancies. We have investigated telomerase activity measured by the telomeric repeat amplification protocol (TRAP) assay and-hTERT levels by real-time RT-PCR in stage I non-small-cell lung carcinomas. The purposes of our study included the comparison of these two techniques in the assessment of telomerase function and the evaluation of their prognostic significance. Telomerase activity and hTERT levels were determined in 90 stage I non-small-cell lung carcinoma patients, using TRAP assay and real-time RT-PCR, respectively. Variables were analyzed by the X2 and Fisher exact tests. Survival was analyzed by the Kaplan-Meier method. Multivariate analysis was performed with the Cox's proportional hazards model. Telomerase activity was elevated in 60 (67%) carcinomas, hTERT was elevated in 43 (48%) carcinomas. Only 21 (23%) tumors had low telomerase function by both TRAP and hTERT expression levels. Telomerase activity and hTERT were significantly correlated (p = 0.017), although 35 cases displayed discordant results. Both telomerase activity and hTERT levels were significantly associated with poor patient overall and disease-free survival (p = 0.019 and p = 0.018 for TRAP, and p = 0.011 and p = 0.012 for hTERT, respectively). Among the 21 patients with tumors displaying low telomerase function, defined by both TRAP and hTERT expression levels, only one succumbed to the disease (p = 0.0053). Our results suggest that the two techniques used in this study evaluate separate aspects of telomerase function and their combination provides powerful prognostic information in lung cancer patients.
| Original language | English |
|---|---|
| Pages (from-to) | 729-736 |
| Number of pages | 8 |
| Journal | Laboratory Investigation |
| Volume | 82 |
| Issue number | 6 |
| Publication status | Published - 2002 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
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Prediction of survival in stage I lung carcinoma patients by telomerase function evaluation. / Marchetti, Antonio; Pellegrini, Caterina; Buttitta, Fiamma; Falleni, Monica; Romagnoli, Solange; Felicioni, Lara; Barassi, Fabio; Salvatore, Simona; Chella, Antonio; Angeletti, Carlo Alberto; Roncalli, Massimo; Coggi, Guido; Bosari, Silvano.
In: Laboratory Investigation, Vol. 82, No. 6, 2002, p. 729-736.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prediction of survival in stage I lung carcinoma patients by telomerase function evaluation
AU - Marchetti, Antonio
AU - Pellegrini, Caterina
AU - Buttitta, Fiamma
AU - Falleni, Monica
AU - Romagnoli, Solange
AU - Felicioni, Lara
AU - Barassi, Fabio
AU - Salvatore, Simona
AU - Chella, Antonio
AU - Angeletti, Carlo Alberto
AU - Roncalli, Massimo
AU - Coggi, Guido
AU - Bosari, Silvano
PY - 2002
Y1 - 2002
N2 - Telomerase activity and telomerase reverse transcriptase (hTERT) expression are elevated in human malignancies. We have investigated telomerase activity measured by the telomeric repeat amplification protocol (TRAP) assay and-hTERT levels by real-time RT-PCR in stage I non-small-cell lung carcinomas. The purposes of our study included the comparison of these two techniques in the assessment of telomerase function and the evaluation of their prognostic significance. Telomerase activity and hTERT levels were determined in 90 stage I non-small-cell lung carcinoma patients, using TRAP assay and real-time RT-PCR, respectively. Variables were analyzed by the X2 and Fisher exact tests. Survival was analyzed by the Kaplan-Meier method. Multivariate analysis was performed with the Cox's proportional hazards model. Telomerase activity was elevated in 60 (67%) carcinomas, hTERT was elevated in 43 (48%) carcinomas. Only 21 (23%) tumors had low telomerase function by both TRAP and hTERT expression levels. Telomerase activity and hTERT were significantly correlated (p = 0.017), although 35 cases displayed discordant results. Both telomerase activity and hTERT levels were significantly associated with poor patient overall and disease-free survival (p = 0.019 and p = 0.018 for TRAP, and p = 0.011 and p = 0.012 for hTERT, respectively). Among the 21 patients with tumors displaying low telomerase function, defined by both TRAP and hTERT expression levels, only one succumbed to the disease (p = 0.0053). Our results suggest that the two techniques used in this study evaluate separate aspects of telomerase function and their combination provides powerful prognostic information in lung cancer patients.
AB - Telomerase activity and telomerase reverse transcriptase (hTERT) expression are elevated in human malignancies. We have investigated telomerase activity measured by the telomeric repeat amplification protocol (TRAP) assay and-hTERT levels by real-time RT-PCR in stage I non-small-cell lung carcinomas. The purposes of our study included the comparison of these two techniques in the assessment of telomerase function and the evaluation of their prognostic significance. Telomerase activity and hTERT levels were determined in 90 stage I non-small-cell lung carcinoma patients, using TRAP assay and real-time RT-PCR, respectively. Variables were analyzed by the X2 and Fisher exact tests. Survival was analyzed by the Kaplan-Meier method. Multivariate analysis was performed with the Cox's proportional hazards model. Telomerase activity was elevated in 60 (67%) carcinomas, hTERT was elevated in 43 (48%) carcinomas. Only 21 (23%) tumors had low telomerase function by both TRAP and hTERT expression levels. Telomerase activity and hTERT were significantly correlated (p = 0.017), although 35 cases displayed discordant results. Both telomerase activity and hTERT levels were significantly associated with poor patient overall and disease-free survival (p = 0.019 and p = 0.018 for TRAP, and p = 0.011 and p = 0.012 for hTERT, respectively). Among the 21 patients with tumors displaying low telomerase function, defined by both TRAP and hTERT expression levels, only one succumbed to the disease (p = 0.0053). Our results suggest that the two techniques used in this study evaluate separate aspects of telomerase function and their combination provides powerful prognostic information in lung cancer patients.
UR - http://www.scopus.com/inward/record.url?scp=0036284709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036284709&partnerID=8YFLogxK
M3 - Article
C2 - 12065683
AN - SCOPUS:0036284709
VL - 82
SP - 729
EP - 736
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 6
ER -