Abstract
Several molecular biomarkers have been suggested as predictors of outcome for pediatric ependymomas but deserve further validation in independent case series. We analyzed intracranial ependymomas belonging to a series of 60 patients prospectively treated according to the protocol sponsored by the Italian Association of Pediatric Hematology-Oncology. We used a tissue microarray to analyze nucleolin (NCL), cyclin-dependent kinase inhibitor 2A (CDKN2A), tumor protein 53 (TP53), and epidermal growth factor receptor (EGFR) by immunohistochemistry and by 1q gain by fluorescent in situ hybridization. The mRNA expression levels of EGFR, human telomerase reverse-transcriptase (HTERT), and Prominin 1 (PROM 1)/CD133 were evaluated by quantitative real-time PCR from cases with fresh-frozen tumor material available. Univariate and multivariate analyses of updated clinical data confirmed the prognostic significance of surgery (P
| Original language | English |
|---|---|
| Pages (from-to) | 1346-1356 |
| Number of pages | 11 |
| Journal | Neuro-Oncology |
| Volume | 14 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2012 |
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Keywords
- biomarker
- EGFR
- ependymoma
- HTERT
- nucleolin
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Clinical Neurology
Cite this
Predictors of outcome in an AIEOP series of childhood ependymomas : A multifactorial analysis. / Modena, Piergiorgio; Buttarelli, Francesca R.; Miceli, Rosalba; Piccinin, Elena; Baldi, Caterina; Antonelli, Manila; Morra, Isabella; Lauriola, Libero; Di Rocco, Concezio; Garrè, Maria Luisa; Sardi, Iacopo; Genitori, Lorenzo; Maestro, Roberta; Gandola, Lorenza; Facchinetti, Federica; Collini, Paola; Sozzi, Gabriella; Giangaspero, Felice; Massimino, Maura.
In: Neuro-Oncology, Vol. 14, No. 11, 11.2012, p. 1346-1356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Predictors of outcome in an AIEOP series of childhood ependymomas
T2 - A multifactorial analysis
AU - Modena, Piergiorgio
AU - Buttarelli, Francesca R.
AU - Miceli, Rosalba
AU - Piccinin, Elena
AU - Baldi, Caterina
AU - Antonelli, Manila
AU - Morra, Isabella
AU - Lauriola, Libero
AU - Di Rocco, Concezio
AU - Garrè, Maria Luisa
AU - Sardi, Iacopo
AU - Genitori, Lorenzo
AU - Maestro, Roberta
AU - Gandola, Lorenza
AU - Facchinetti, Federica
AU - Collini, Paola
AU - Sozzi, Gabriella
AU - Giangaspero, Felice
AU - Massimino, Maura
PY - 2012/11
Y1 - 2012/11
N2 - Several molecular biomarkers have been suggested as predictors of outcome for pediatric ependymomas but deserve further validation in independent case series. We analyzed intracranial ependymomas belonging to a series of 60 patients prospectively treated according to the protocol sponsored by the Italian Association of Pediatric Hematology-Oncology. We used a tissue microarray to analyze nucleolin (NCL), cyclin-dependent kinase inhibitor 2A (CDKN2A), tumor protein 53 (TP53), and epidermal growth factor receptor (EGFR) by immunohistochemistry and by 1q gain by fluorescent in situ hybridization. The mRNA expression levels of EGFR, human telomerase reverse-transcriptase (HTERT), and Prominin 1 (PROM 1)/CD133 were evaluated by quantitative real-time PCR from cases with fresh-frozen tumor material available. Univariate and multivariate analyses of updated clinical data confirmed the prognostic significance of surgery (P
AB - Several molecular biomarkers have been suggested as predictors of outcome for pediatric ependymomas but deserve further validation in independent case series. We analyzed intracranial ependymomas belonging to a series of 60 patients prospectively treated according to the protocol sponsored by the Italian Association of Pediatric Hematology-Oncology. We used a tissue microarray to analyze nucleolin (NCL), cyclin-dependent kinase inhibitor 2A (CDKN2A), tumor protein 53 (TP53), and epidermal growth factor receptor (EGFR) by immunohistochemistry and by 1q gain by fluorescent in situ hybridization. The mRNA expression levels of EGFR, human telomerase reverse-transcriptase (HTERT), and Prominin 1 (PROM 1)/CD133 were evaluated by quantitative real-time PCR from cases with fresh-frozen tumor material available. Univariate and multivariate analyses of updated clinical data confirmed the prognostic significance of surgery (P
KW - biomarker
KW - EGFR
KW - ependymoma
KW - HTERT
KW - nucleolin
UR - http://www.scopus.com/inward/record.url?scp=84868018309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868018309&partnerID=8YFLogxK
U2 - 10.1093/neuonc/nos245
DO - 10.1093/neuonc/nos245
M3 - Article
C2 - 23076205
AN - SCOPUS:84868018309
VL - 14
SP - 1346
EP - 1356
JO - Neuro-Oncology
JF - Neuro-Oncology
SN - 1522-8517
IS - 11
ER -