Predictors of outcome in an AIEOP series of childhood ependymomas: A multifactorial analysis

Piergiorgio Modena, Francesca R. Buttarelli, Rosalba Miceli, Elena Piccinin, Caterina Baldi, Manila Antonelli, Isabella Morra, Libero Lauriola, Concezio Di Rocco, Maria Luisa Garrè, Iacopo Sardi, Lorenzo Genitori, Roberta Maestro, Lorenza Gandola, Federica Facchinetti, Paola Collini, Gabriella Sozzi, Felice Giangaspero, Maura Massimino

Research output: Contribution to journalArticle

Abstract

Several molecular biomarkers have been suggested as predictors of outcome for pediatric ependymomas but deserve further validation in independent case series. We analyzed intracranial ependymomas belonging to a series of 60 patients prospectively treated according to the protocol sponsored by the Italian Association of Pediatric Hematology-Oncology. We used a tissue microarray to analyze nucleolin (NCL), cyclin-dependent kinase inhibitor 2A (CDKN2A), tumor protein 53 (TP53), and epidermal growth factor receptor (EGFR) by immunohistochemistry and by 1q gain by fluorescent in situ hybridization. The mRNA expression levels of EGFR, human telomerase reverse-transcriptase (HTERT), and Prominin 1 (PROM 1)/CD133 were evaluated by quantitative real-time PCR from cases with fresh-frozen tumor material available. Univariate and multivariate analyses of updated clinical data confirmed the prognostic significance of surgery (P

Original languageEnglish
Pages (from-to)1346-1356
Number of pages11
JournalNeuro-Oncology
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 2012

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Ependymoma
Epidermal Growth Factor Receptor
Tissue Array Analysis
Cyclin-Dependent Kinase Inhibitor p16
Pediatrics
Hematology
Fluorescence In Situ Hybridization
Real-Time Polymerase Chain Reaction
Neoplasms
Multivariate Analysis
Biomarkers
Immunohistochemistry
Messenger RNA
Proteins
Familial ependymoma
human TERT protein
AC133 Antigen
nucleolin

Keywords

  • biomarker
  • EGFR
  • ependymoma
  • HTERT
  • nucleolin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Predictors of outcome in an AIEOP series of childhood ependymomas : A multifactorial analysis. / Modena, Piergiorgio; Buttarelli, Francesca R.; Miceli, Rosalba; Piccinin, Elena; Baldi, Caterina; Antonelli, Manila; Morra, Isabella; Lauriola, Libero; Di Rocco, Concezio; Garrè, Maria Luisa; Sardi, Iacopo; Genitori, Lorenzo; Maestro, Roberta; Gandola, Lorenza; Facchinetti, Federica; Collini, Paola; Sozzi, Gabriella; Giangaspero, Felice; Massimino, Maura.

In: Neuro-Oncology, Vol. 14, No. 11, 11.2012, p. 1346-1356.

Research output: Contribution to journalArticle

Modena, P, Buttarelli, FR, Miceli, R, Piccinin, E, Baldi, C, Antonelli, M, Morra, I, Lauriola, L, Di Rocco, C, Garrè, ML, Sardi, I, Genitori, L, Maestro, R, Gandola, L, Facchinetti, F, Collini, P, Sozzi, G, Giangaspero, F & Massimino, M 2012, 'Predictors of outcome in an AIEOP series of childhood ependymomas: A multifactorial analysis', Neuro-Oncology, vol. 14, no. 11, pp. 1346-1356. https://doi.org/10.1093/neuonc/nos245
Modena, Piergiorgio ; Buttarelli, Francesca R. ; Miceli, Rosalba ; Piccinin, Elena ; Baldi, Caterina ; Antonelli, Manila ; Morra, Isabella ; Lauriola, Libero ; Di Rocco, Concezio ; Garrè, Maria Luisa ; Sardi, Iacopo ; Genitori, Lorenzo ; Maestro, Roberta ; Gandola, Lorenza ; Facchinetti, Federica ; Collini, Paola ; Sozzi, Gabriella ; Giangaspero, Felice ; Massimino, Maura. / Predictors of outcome in an AIEOP series of childhood ependymomas : A multifactorial analysis. In: Neuro-Oncology. 2012 ; Vol. 14, No. 11. pp. 1346-1356.
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