Predominant T(H1) cytokine pattern in peripheral blood from subjects with chronic obstructive pulmonary disease

M. Majori, M. Corradi, A. Caminati, G. Cacciani, S. Bertacco, A. Pesci

Research output: Contribution to journalArticle

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Abstract

Background: Previous studies have provided evidence for an inflammatory process in the large airways of subjects with chronic obstructive pulmonary disease (COPD), consisting predominantly of activated T cells. No data are available on the T(H1)/T(H2) T-cell cytokine pattern in this disease. Objective: We sought to characterize the T(H1)/T(H2) T-cell cytokine pattern in subjects with COPD. Methods: We examined the IFN-γ and IL-4 expression in peripheral blood CD4+ and CD8+ T cells from 20 patients with COPD and 25 control subjects by using a flow cytometric method of intracellular cytokine detection. We also examined the expression of 2 surface activation markers (CD25 and HLA-DR) on peripheral blood CD4+ and CD8+ T cells. Results: There was an increased percentage of IFN-γ-producing cells (30.3% [range, 12.9% to 60.4%] vs 19.1% [range, 4% to 31.2%], P = .003) and a decreased percentage of IL-4-producing cells (4.55% [range, 0.6% to 11.3%] vs 9.5% [2.1% to 21.3%], P = .0008) among peripheral blood CD4+ T cells from the patients with COPD compared with control subjects. There was no significant difference between the 2 groups in the percentage of peripheral blood CD8+ T cells producing IFN-γ or IL-4 or in the percentage of peripheral blood CD4+ and CD8+ T cells expressing CD25 and HLA-DR. Conclusion: These findings provide evidence for a T(H1)-like immune response of peripheral blood CD4+ T cells in subjects with COPD.

Original languageEnglish
Pages (from-to)458-462
Number of pages5
JournalJournal of Allergy and Clinical Immunology
Volume103
Issue number3 I
Publication statusPublished - 1999

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Chronic Obstructive Pulmonary Disease
Cytokines
T-Lymphocytes
Interleukin-4
HLA-DR Antigens

Keywords

  • Chronic obstructive pulmonary disease
  • Cytokines
  • Lymphocytes
  • Peripheral blood

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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Predominant T(H1) cytokine pattern in peripheral blood from subjects with chronic obstructive pulmonary disease. / Majori, M.; Corradi, M.; Caminati, A.; Cacciani, G.; Bertacco, S.; Pesci, A.

In: Journal of Allergy and Clinical Immunology, Vol. 103, No. 3 I, 1999, p. 458-462.

Research output: Contribution to journalArticle

Majori, M. ; Corradi, M. ; Caminati, A. ; Cacciani, G. ; Bertacco, S. ; Pesci, A. / Predominant T(H1) cytokine pattern in peripheral blood from subjects with chronic obstructive pulmonary disease. In: Journal of Allergy and Clinical Immunology. 1999 ; Vol. 103, No. 3 I. pp. 458-462.
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AU - Corradi, M.

AU - Caminati, A.

AU - Cacciani, G.

AU - Bertacco, S.

AU - Pesci, A.

PY - 1999

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N2 - Background: Previous studies have provided evidence for an inflammatory process in the large airways of subjects with chronic obstructive pulmonary disease (COPD), consisting predominantly of activated T cells. No data are available on the T(H1)/T(H2) T-cell cytokine pattern in this disease. Objective: We sought to characterize the T(H1)/T(H2) T-cell cytokine pattern in subjects with COPD. Methods: We examined the IFN-γ and IL-4 expression in peripheral blood CD4+ and CD8+ T cells from 20 patients with COPD and 25 control subjects by using a flow cytometric method of intracellular cytokine detection. We also examined the expression of 2 surface activation markers (CD25 and HLA-DR) on peripheral blood CD4+ and CD8+ T cells. Results: There was an increased percentage of IFN-γ-producing cells (30.3% [range, 12.9% to 60.4%] vs 19.1% [range, 4% to 31.2%], P = .003) and a decreased percentage of IL-4-producing cells (4.55% [range, 0.6% to 11.3%] vs 9.5% [2.1% to 21.3%], P = .0008) among peripheral blood CD4+ T cells from the patients with COPD compared with control subjects. There was no significant difference between the 2 groups in the percentage of peripheral blood CD8+ T cells producing IFN-γ or IL-4 or in the percentage of peripheral blood CD4+ and CD8+ T cells expressing CD25 and HLA-DR. Conclusion: These findings provide evidence for a T(H1)-like immune response of peripheral blood CD4+ T cells in subjects with COPD.

AB - Background: Previous studies have provided evidence for an inflammatory process in the large airways of subjects with chronic obstructive pulmonary disease (COPD), consisting predominantly of activated T cells. No data are available on the T(H1)/T(H2) T-cell cytokine pattern in this disease. Objective: We sought to characterize the T(H1)/T(H2) T-cell cytokine pattern in subjects with COPD. Methods: We examined the IFN-γ and IL-4 expression in peripheral blood CD4+ and CD8+ T cells from 20 patients with COPD and 25 control subjects by using a flow cytometric method of intracellular cytokine detection. We also examined the expression of 2 surface activation markers (CD25 and HLA-DR) on peripheral blood CD4+ and CD8+ T cells. Results: There was an increased percentage of IFN-γ-producing cells (30.3% [range, 12.9% to 60.4%] vs 19.1% [range, 4% to 31.2%], P = .003) and a decreased percentage of IL-4-producing cells (4.55% [range, 0.6% to 11.3%] vs 9.5% [2.1% to 21.3%], P = .0008) among peripheral blood CD4+ T cells from the patients with COPD compared with control subjects. There was no significant difference between the 2 groups in the percentage of peripheral blood CD8+ T cells producing IFN-γ or IL-4 or in the percentage of peripheral blood CD4+ and CD8+ T cells expressing CD25 and HLA-DR. Conclusion: These findings provide evidence for a T(H1)-like immune response of peripheral blood CD4+ T cells in subjects with COPD.

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