Preferential pairing of T and B cells for production of antibodies without covalent association of T and B epitopes

Matteo Bellone, Peter I. Karachunski, Norma Ostlie, Sijin Lei, Bianca M. Conti-Tronconi

Research output: Contribution to journalArticle

Abstract

T cell from H-2b mice recognize at least 12 sequence regions on the Torpedo acetylcholine receptor (TAChR) α, γ and δ subunits. Immunization of C57BL/6 mice with individual synthetic TAChR sequences known to contain CD4+ epitopes resulted in most cases (10 out of 12 peptides) in anti-peptide antibody (Ab) production, indicating that short TAChR sequences contain both CD4+ and B epitopes. Immunization of C57BL/6 mice with a mixture of a CD4+ epitope peptide, from the TAChR or from an unrelated protein, plus another TAChR sequence forming a 'pure' B epitope (Tα63-80), induced in most cases anti-peptide Ab and CD4+ cell sensitization only against the peptide containing the CD4+ epitope. However, when the T epitope peptide Tα360-378 was co-injected with the B epitope, Ab were also produced against the B epitope peptide. Injection of the individual peptides Tα360-378 and Tα63-80 at different and distant sites along the back of mice elicited sensitization of CD4+ cells and Ab production only against peptide Tα360-378. Therefore, when optimal cooperation between T and B cells occurs, spatial proximity but not covalent association of the B and the CD4+ epitope is necessary for production of Ab against the B epitope.

Original languageEnglish
Pages (from-to)799-804
Number of pages6
JournalEuropean Journal of Immunology
Volume24
Issue number4
Publication statusPublished - Apr 1994

Keywords

  • Experimental autoimmune myasthenia gravis
  • Nicotinic acetylcholine receptor
  • Synthetic peptides
  • T and B epitopes
  • T-B cell cooperation

ASJC Scopus subject areas

  • Immunology

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