TY - JOUR
T1 - Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres
AU - Mulder, Lubbertus C.
AU - Sacco, Maria G.
AU - Mangiarini, Laura
AU - Brown, John
AU - Collotta, Angelo
AU - Villa, Anna
AU - De Giovanni, Anna M.
AU - Vezzoni, Paolo
AU - Clerici, Libero
PY - 1993
Y1 - 1993
N2 - Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.
AB - Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.
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U2 - 10.1016/1050-3862(93)90010-G
DO - 10.1016/1050-3862(93)90010-G
M3 - Article
C2 - 8043307
AN - SCOPUS:43949174212
VL - 10
SP - 147
EP - 149
JO - Genetic Analysis - Biomolecular Engineering
JF - Genetic Analysis - Biomolecular Engineering
SN - 1050-3862
IS - 6
ER -