Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres

Lubbertus C. Mulder, Maria G. Sacco, Laura Mangiarini, John Brown, Angelo Collotta, Anna Villa, Anna M. De Giovanni, Paolo Vezzoni, Libero Clerici

Research output: Contribution to journalArticlepeer-review

Abstract

Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.

Original languageEnglish
Pages (from-to)147-149
Number of pages3
JournalGenetic Analysis: Biomolecular Engineering
Volume10
Issue number6
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

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