Prelamin A is involved in early steps of muscle differentiation

Cristina Capanni; Rosalba Del Coco; Stefano Squarzoni; Marta Columbaro; Elisabetta Mattioli; Daria Camozzi; Anna Rocchi; Katia Scotlandi; Nadir Maraldi; Roland Foisner; Giovanna Lattanzi

doi:10.1016/j.yexcr.2008.09.026

Prelamin A is involved in early steps of muscle differentiation

Cristina Capanni, Rosalba Del Coco, Stefano Squarzoni, Marta Columbaro, Elisabetta Mattioli, Daria Camozzi, Anna Rocchi, Katia Scotlandi, Nadir Maraldi, Roland Foisner, Giovanna Lattanzi

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2α were observed. Furthermore, prelamin A was found in a complex with LAP2α in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2α and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.

Original language	English
Pages (from-to)	3628-3637
Number of pages	10
Journal	Experimental Cell Research
Volume	314
Issue number	20
DOIs	https://doi.org/10.1016/j.yexcr.2008.09.026
Publication status	Published - Dec 10 2008

Keywords

Caveolin 3
LAP2 alpha
Myoblast differentiation
Prelamin A

ASJC Scopus subject areas

Cell Biology

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Prelamin A is involved in early steps of muscle differentiation. / Capanni, Cristina; Del Coco, Rosalba; Squarzoni, Stefano ; Columbaro, Marta; Mattioli, Elisabetta; Camozzi, Daria; Rocchi, Anna; Scotlandi, Katia; Maraldi, Nadir; Foisner, Roland; Lattanzi, Giovanna.

In: Experimental Cell Research, Vol. 314, No. 20, 10.12.2008, p. 3628-3637.

Research output: Contribution to journal › Article › peer-review

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abstract = "Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2α were observed. Furthermore, prelamin A was found in a complex with LAP2α in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2α and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.",

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author = "Cristina Capanni and {Del Coco}, Rosalba and Stefano Squarzoni and Marta Columbaro and Elisabetta Mattioli and Daria Camozzi and Anna Rocchi and Katia Scotlandi and Nadir Maraldi and Roland Foisner and Giovanna Lattanzi",

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AU - Capanni, Cristina

AU - Del Coco, Rosalba

AU - Squarzoni, Stefano

AU - Columbaro, Marta

AU - Mattioli, Elisabetta

AU - Camozzi, Daria

AU - Rocchi, Anna

AU - Scotlandi, Katia

AU - Maraldi, Nadir

AU - Foisner, Roland

AU - Lattanzi, Giovanna

PY - 2008/12/10

Y1 - 2008/12/10

N2 - Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2α were observed. Furthermore, prelamin A was found in a complex with LAP2α in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2α and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.

AB - Lamin A is a nuclear lamina constituent implicated in a number of human disorders including Emery-Dreifuss muscular dystrophy. Since increasing evidence suggests a role of the lamin A precursor in nuclear functions, we investigated the processing of prelamin A during differentiation of C2C12 mouse myoblasts. We show that both protein levels and cellular localization of prelamin A are modulated during myoblast activation. Similar changes of lamin A-binding proteins emerin and LAP2α were observed. Furthermore, prelamin A was found in a complex with LAP2α in differentiating myoblasts. Prelamin A accumulation in cycling myoblasts by expressing unprocessable mutants affected LAP2α and PCNA amount and increased caveolin 3 mRNA and protein levels, while accumulation of prelamin A in differentiated muscle cells following treatment with a farnesyl transferase inhibitor appeared to inhibit caveolin 3 expression. Our data provide evidence for a critical role of the lamin A precursor in the early steps of muscle cell differentiation.

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KW - LAP2 alpha

KW - Myoblast differentiation

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