Preliminary characterization of N-trimethylchitosan as a nanocarrier for malaria vaccine

P. O. Nnamani; G. Scoles; S. Kröl

Preliminary characterization of N-trimethylchitosan as a nanocarrier for malaria vaccine

P. O. Nnamani, G. Scoles, S. Kröl

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

Background & objectives: With the current snags from the use of Artemisinin-combination therapies (ACTs) in malaria treatment in addition to fear of cross-resistance to unrelated drugs, raising the immunocompetence of individuals in malaria endemic areas by vaccination is the best approach to malaria-free world. Methods: Water-soluble cationic derivative, N, N, N-trimethylchitosan (TMC) was synthesized from chitosan. Nanoparticles of the TMC were prepared in various media [milliQ water, Na₂CO₃ (pH 10.92), Na₂HPO₄ (PBS, pH 9.01 and alhydrogel®] which were characterized as adjuvants for possible vaccine delivery. The nanoparticles were characterized for particle size, surface charge and morphology using microscopy (Phase contrast microscope and Confocal laser scanning microscope), and Malvern zetasizer Nano-ZS. Time-resolved particle size analysis was performed after one month storage of the TMC nanoparticles at 4°C. Results: The result of the study showed that PBS was the best medium that produced cationic, monodispersed and stable TMC nanoparticles of

Original language	English
Pages (from-to)	224-230
Number of pages	7
Journal	Journal of Vector Borne Diseases
Volume	48
Issue number	4
Publication status	Published - Dec 2011

Keywords

Alhydrogel®
Malaria
N-trimethylchitosan
Nanocarrier
PBS
Vaccine

ASJC Scopus subject areas

Infectious Diseases
Parasitology

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abstract = "Background & objectives: With the current snags from the use of Artemisinin-combination therapies (ACTs) in malaria treatment in addition to fear of cross-resistance to unrelated drugs, raising the immunocompetence of individuals in malaria endemic areas by vaccination is the best approach to malaria-free world. Methods: Water-soluble cationic derivative, N, N, N-trimethylchitosan (TMC) was synthesized from chitosan. Nanoparticles of the TMC were prepared in various media [milliQ water, Na2CO3 (pH 10.92), Na2HPO4 (PBS, pH 9.01 and alhydrogel{\textregistered}] which were characterized as adjuvants for possible vaccine delivery. The nanoparticles were characterized for particle size, surface charge and morphology using microscopy (Phase contrast microscope and Confocal laser scanning microscope), and Malvern zetasizer Nano-ZS. Time-resolved particle size analysis was performed after one month storage of the TMC nanoparticles at 4°C. Results: The result of the study showed that PBS was the best medium that produced cationic, monodispersed and stable TMC nanoparticles of ",

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year = "2011",

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AU - Scoles, G.

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