Preliminary evidence for high anti-PLAC1 antibody levels in infertile patients with repeated unexplained implantation failure

M. Matteo, P. Greco, P. E. Levi Setti, E. Morenghi, F. De Rosario, F. Massenzio, E. Albani, P. Totaro, A. Liso

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: Placenta-specific1 (PLAC1) is a trophoblast-specific gene encoding for a protein that is highly expressed in human placenta, on the surface of the syncytiotrophoblast. PLAC1 was found to elicit spontaneous antibody responses in cancer patients. We aimed to determine the levels of anti-PLAC1 antibodies in infertile women with a history of unexplained repeated implantation failure after IVF cycles as compared to fertile women. Study design: An observational case-control clinical study. Main outcome measure(s): Two groups of patients were analysed in two different experimental settings: 21 infertile women and 81 control patients were enrolled in the first group, 16 infertile women and 67 fertile controls in the second group. Anti-PLAC1 antibody levels and ranking were analysed by ELISA test. Results: In both groups of infertile patients enrolled, optical densities (OD) from ELISA test ranked significantly higher than those of controls (0.27 ± 0.2 vs. 0.13 ± 0.1 respectively; p = 0.0009 in the first group), (0.62 ± 0.38 vs. 0.39 ± 0.35 respectively; p = 0.0044 in the second experiment). In the first group about one case in four (29%) had OD levels above the 95thpercentile (0.337) for healthy controls (p = 0.005). In the second experiment 4 out of 16 cases (25%) had OD levels above the 95th percentile (0.878) for healthy controls (p = 0.023). Conclusions: Anti-PLAC1 antibodies could represent a biomarker associated with infertility and with high probability of repeated implantation failure after ovarian stimulation and IVF-ET, greatly improving the diagnostic work up of infertile couples.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalPlacenta
Volume34
Issue number4
DOIs
Publication statusPublished - Apr 2013

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Placenta
Antibodies
Trophoblasts
Enzyme-Linked Immunosorbent Assay
Ovulation Induction
Infertility
Antibody Formation
Case-Control Studies
Biomarkers
Outcome Assessment (Health Care)
Genes
Neoplasms
Proteins

Keywords

  • Autoantibodies
  • Implantation failure
  • In vitro fertilization
  • Infertility
  • PLAC1

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Preliminary evidence for high anti-PLAC1 antibody levels in infertile patients with repeated unexplained implantation failure. / Matteo, M.; Greco, P.; Levi Setti, P. E.; Morenghi, E.; De Rosario, F.; Massenzio, F.; Albani, E.; Totaro, P.; Liso, A.

In: Placenta, Vol. 34, No. 4, 04.2013, p. 335-339.

Research output: Contribution to journalArticle

Matteo, M, Greco, P, Levi Setti, PE, Morenghi, E, De Rosario, F, Massenzio, F, Albani, E, Totaro, P & Liso, A 2013, 'Preliminary evidence for high anti-PLAC1 antibody levels in infertile patients with repeated unexplained implantation failure', Placenta, vol. 34, no. 4, pp. 335-339. https://doi.org/10.1016/j.placenta.2013.01.006
Matteo, M. ; Greco, P. ; Levi Setti, P. E. ; Morenghi, E. ; De Rosario, F. ; Massenzio, F. ; Albani, E. ; Totaro, P. ; Liso, A. / Preliminary evidence for high anti-PLAC1 antibody levels in infertile patients with repeated unexplained implantation failure. In: Placenta. 2013 ; Vol. 34, No. 4. pp. 335-339.
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abstract = "Objective: Placenta-specific1 (PLAC1) is a trophoblast-specific gene encoding for a protein that is highly expressed in human placenta, on the surface of the syncytiotrophoblast. PLAC1 was found to elicit spontaneous antibody responses in cancer patients. We aimed to determine the levels of anti-PLAC1 antibodies in infertile women with a history of unexplained repeated implantation failure after IVF cycles as compared to fertile women. Study design: An observational case-control clinical study. Main outcome measure(s): Two groups of patients were analysed in two different experimental settings: 21 infertile women and 81 control patients were enrolled in the first group, 16 infertile women and 67 fertile controls in the second group. Anti-PLAC1 antibody levels and ranking were analysed by ELISA test. Results: In both groups of infertile patients enrolled, optical densities (OD) from ELISA test ranked significantly higher than those of controls (0.27 ± 0.2 vs. 0.13 ± 0.1 respectively; p = 0.0009 in the first group), (0.62 ± 0.38 vs. 0.39 ± 0.35 respectively; p = 0.0044 in the second experiment). In the first group about one case in four (29{\%}) had OD levels above the 95thpercentile (0.337) for healthy controls (p = 0.005). In the second experiment 4 out of 16 cases (25{\%}) had OD levels above the 95th percentile (0.878) for healthy controls (p = 0.023). Conclusions: Anti-PLAC1 antibodies could represent a biomarker associated with infertility and with high probability of repeated implantation failure after ovarian stimulation and IVF-ET, greatly improving the diagnostic work up of infertile couples.",
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AU - Matteo, M.

AU - Greco, P.

AU - Levi Setti, P. E.

AU - Morenghi, E.

AU - De Rosario, F.

AU - Massenzio, F.

AU - Albani, E.

AU - Totaro, P.

AU - Liso, A.

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N2 - Objective: Placenta-specific1 (PLAC1) is a trophoblast-specific gene encoding for a protein that is highly expressed in human placenta, on the surface of the syncytiotrophoblast. PLAC1 was found to elicit spontaneous antibody responses in cancer patients. We aimed to determine the levels of anti-PLAC1 antibodies in infertile women with a history of unexplained repeated implantation failure after IVF cycles as compared to fertile women. Study design: An observational case-control clinical study. Main outcome measure(s): Two groups of patients were analysed in two different experimental settings: 21 infertile women and 81 control patients were enrolled in the first group, 16 infertile women and 67 fertile controls in the second group. Anti-PLAC1 antibody levels and ranking were analysed by ELISA test. Results: In both groups of infertile patients enrolled, optical densities (OD) from ELISA test ranked significantly higher than those of controls (0.27 ± 0.2 vs. 0.13 ± 0.1 respectively; p = 0.0009 in the first group), (0.62 ± 0.38 vs. 0.39 ± 0.35 respectively; p = 0.0044 in the second experiment). In the first group about one case in four (29%) had OD levels above the 95thpercentile (0.337) for healthy controls (p = 0.005). In the second experiment 4 out of 16 cases (25%) had OD levels above the 95th percentile (0.878) for healthy controls (p = 0.023). Conclusions: Anti-PLAC1 antibodies could represent a biomarker associated with infertility and with high probability of repeated implantation failure after ovarian stimulation and IVF-ET, greatly improving the diagnostic work up of infertile couples.

AB - Objective: Placenta-specific1 (PLAC1) is a trophoblast-specific gene encoding for a protein that is highly expressed in human placenta, on the surface of the syncytiotrophoblast. PLAC1 was found to elicit spontaneous antibody responses in cancer patients. We aimed to determine the levels of anti-PLAC1 antibodies in infertile women with a history of unexplained repeated implantation failure after IVF cycles as compared to fertile women. Study design: An observational case-control clinical study. Main outcome measure(s): Two groups of patients were analysed in two different experimental settings: 21 infertile women and 81 control patients were enrolled in the first group, 16 infertile women and 67 fertile controls in the second group. Anti-PLAC1 antibody levels and ranking were analysed by ELISA test. Results: In both groups of infertile patients enrolled, optical densities (OD) from ELISA test ranked significantly higher than those of controls (0.27 ± 0.2 vs. 0.13 ± 0.1 respectively; p = 0.0009 in the first group), (0.62 ± 0.38 vs. 0.39 ± 0.35 respectively; p = 0.0044 in the second experiment). In the first group about one case in four (29%) had OD levels above the 95thpercentile (0.337) for healthy controls (p = 0.005). In the second experiment 4 out of 16 cases (25%) had OD levels above the 95th percentile (0.878) for healthy controls (p = 0.023). Conclusions: Anti-PLAC1 antibodies could represent a biomarker associated with infertility and with high probability of repeated implantation failure after ovarian stimulation and IVF-ET, greatly improving the diagnostic work up of infertile couples.

KW - Autoantibodies

KW - Implantation failure

KW - In vitro fertilization

KW - Infertility

KW - PLAC1

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