Introduction: the study of the human microbiome is one of the most dynamic current topics in biomedical research. A significantchallenge in this field is the development of cost effective method for a robust interrogation of microbiota composition.Advances in next-generation sequencing (NGS) technologies have allowed for efficient and molecular-based analysisof microbial communities. Association between diseases and imbalance of the microbial populations are today wellinvestigated. Methods: Pemphigus Vulgaris (PV) and Bullous Pemphigoid (BP) are two rare autoantibody-mediated blisteringskin diseases. In this pilot study, we characterized the intestinal, cutaneous and oral mucosal microbiota compositionsin PV/BP patients, in order to evaluate the potential role of the bacterial composition in these dermatologicaldisorders. Particularly, we performed a high-throughput sequencing analysis of the V3-V4 hypervariable regions of16S rRNA for the evaluation of bacterial composition of stool, skin and oral mucosa samples in PV (n=12) and BP(n=8) patients. Results: a similar composition of the intestinal microbiota was observed in PV and BP patients. The evaluationof skin lesions revealed a prevalence of Firmicutes phylum in both patients’ groups. In the cutaneous microbiota, weidentified a significant decreased abundance of Bacteroidetes phylum compared to healthy controls. Conclusions: the results obtained from our standardized NGS pipeline, reinforced by correlation with other clinical and biochemicalparameters, will contribute to clarify the mechanisms of these rare diseases.
|Translated title of the contribution||Preliminary study of the microbiome in the gut, skin and oral mucosa of patients affected by Pemphigus Vulgaris and Bullous Pemphigoid.|
|Publication status||Published - Jan 1 2019|
ASJC Scopus subject areas
- Clinical Biochemistry
- Medical Laboratory Technology
- Biochemistry, medical