Studio preliminare del microbiota intestinale, cutaneo e della mucosa orale in pazienti affetti da Pemfigo Volgare e Pemfigoide Bolloso

Translated title of the contribution: Preliminary study of the microbiome in the gut, skin and oral mucosa of patients affected by Pemphigus Vulgaris and Bullous Pemphigoid.

Elisa de Paolis, Giovanni Luca Scaglione, Maria de Bonis, Luca Fania, Flavio Mignone, Ettore Capoluongo

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: the study of the human microbiome is one of the most dynamic current topics in biomedical research. A significantchallenge in this field is the development of cost effective method for a robust interrogation of microbiota composition.Advances in next-generation sequencing (NGS) technologies have allowed for efficient and molecular-based analysisof microbial communities. Association between diseases and imbalance of the microbial populations are today wellinvestigated. Methods: Pemphigus Vulgaris (PV) and Bullous Pemphigoid (BP) are two rare autoantibody-mediated blisteringskin diseases. In this pilot study, we characterized the intestinal, cutaneous and oral mucosal microbiota compositionsin PV/BP patients, in order to evaluate the potential role of the bacterial composition in these dermatologicaldisorders. Particularly, we performed a high-throughput sequencing analysis of the V3-V4 hypervariable regions of16S rRNA for the evaluation of bacterial composition of stool, skin and oral mucosa samples in PV (n=12) and BP(n=8) patients. Results: a similar composition of the intestinal microbiota was observed in PV and BP patients. The evaluationof skin lesions revealed a prevalence of Firmicutes phylum in both patients’ groups. In the cutaneous microbiota, weidentified a significant decreased abundance of Bacteroidetes phylum compared to healthy controls. Conclusions: the results obtained from our standardized NGS pipeline, reinforced by correlation with other clinical and biochemicalparameters, will contribute to clarify the mechanisms of these rare diseases.

Translated title of the contributionPreliminary study of the microbiome in the gut, skin and oral mucosa of patients affected by Pemphigus Vulgaris and Bullous Pemphigoid.
Original languageItalian
JournalBiochimica Clinica
Volume43
Issue number4
DOIs
Publication statusPublished - Jan 1 2019

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical

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