Prenatal diagnosis of congenital varicella infection

Alessandra Kustermann, Cinzia Zoppini, Beatrice Tassis, Marzia Della Morte, Giuseppe Colucci, Umberto Nicolini

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fourteen fetuses at risk of Varicella-Zoster virus (VZV) infection underwent prenatal diagnosis at 10-24 weeks' gestation by a combination of chorionic villus sampling, amniocentesis, and fetal blood sampling. Polymerase chain reaction (PCR) was done on fetal and placental tissues, using primers which define a 221 bp region of the gene coding for the 44 kD protein of VZV. Positive cases were further analysed by dot blot hybridization, using radiolabelled DNA probes corresponding to the Hind III fragment VZV genome. The rate of placental/fetal infection was 36 per cent (5/14 fetuses: 2/11 in the first and 3/3 in the second trimester). At post-mortem examination, two aborted fetuses had hydrocephaly and VZV DNA was found in most of the examined tissues. The nine women who tested negative at prenatal investigation delivered healthy neonates whose VZV-specific IgM antibody titres were negative and none of them developed herpes tester infection. In view of the high frequency of fetal VZV infection and the reported low rate of malformations, the role of invasive prenatal diagnosis in women who acquire the infection in the first half of gestation is mainly that of reassurance when the test is negative.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalPrenatal Diagnosis
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1996

Fingerprint

Human Herpesvirus 3
Chickenpox
Prenatal Diagnosis
Infection
Fetus
Virus Diseases
Aborted Fetus
Chorionic Villi Sampling
Pregnancy
Amniocentesis
DNA Probes
Second Pregnancy Trimester
Hydrocephalus
Fetal Blood
Immunoglobulin M
Autopsy
Newborn Infant
Genome
Polymerase Chain Reaction
Antibodies

Keywords

  • Polymerase chain reaction
  • Prenatal diagnosis
  • Varicella infection

ASJC Scopus subject areas

  • Genetics(clinical)
  • Obstetrics and Gynaecology

Cite this

Prenatal diagnosis of congenital varicella infection. / Kustermann, Alessandra; Zoppini, Cinzia; Tassis, Beatrice; Della Morte, Marzia; Colucci, Giuseppe; Nicolini, Umberto.

In: Prenatal Diagnosis, Vol. 16, No. 1, 01.1996, p. 71-74.

Research output: Contribution to journalArticle

Kustermann, Alessandra ; Zoppini, Cinzia ; Tassis, Beatrice ; Della Morte, Marzia ; Colucci, Giuseppe ; Nicolini, Umberto. / Prenatal diagnosis of congenital varicella infection. In: Prenatal Diagnosis. 1996 ; Vol. 16, No. 1. pp. 71-74.
@article{043e30fd7f964815944975a8785bd5a9,
title = "Prenatal diagnosis of congenital varicella infection",
abstract = "Fourteen fetuses at risk of Varicella-Zoster virus (VZV) infection underwent prenatal diagnosis at 10-24 weeks' gestation by a combination of chorionic villus sampling, amniocentesis, and fetal blood sampling. Polymerase chain reaction (PCR) was done on fetal and placental tissues, using primers which define a 221 bp region of the gene coding for the 44 kD protein of VZV. Positive cases were further analysed by dot blot hybridization, using radiolabelled DNA probes corresponding to the Hind III fragment VZV genome. The rate of placental/fetal infection was 36 per cent (5/14 fetuses: 2/11 in the first and 3/3 in the second trimester). At post-mortem examination, two aborted fetuses had hydrocephaly and VZV DNA was found in most of the examined tissues. The nine women who tested negative at prenatal investigation delivered healthy neonates whose VZV-specific IgM antibody titres were negative and none of them developed herpes tester infection. In view of the high frequency of fetal VZV infection and the reported low rate of malformations, the role of invasive prenatal diagnosis in women who acquire the infection in the first half of gestation is mainly that of reassurance when the test is negative.",
keywords = "Polymerase chain reaction, Prenatal diagnosis, Varicella infection",
author = "Alessandra Kustermann and Cinzia Zoppini and Beatrice Tassis and {Della Morte}, Marzia and Giuseppe Colucci and Umberto Nicolini",
year = "1996",
month = "1",
doi = "10.1002/(SICI)1097-0223(199601)16:1<71::AID-PD806>3.0.CO;2-Z",
language = "English",
volume = "16",
pages = "71--74",
journal = "Prenatal Diagnosis",
issn = "0197-3851",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Prenatal diagnosis of congenital varicella infection

AU - Kustermann, Alessandra

AU - Zoppini, Cinzia

AU - Tassis, Beatrice

AU - Della Morte, Marzia

AU - Colucci, Giuseppe

AU - Nicolini, Umberto

PY - 1996/1

Y1 - 1996/1

N2 - Fourteen fetuses at risk of Varicella-Zoster virus (VZV) infection underwent prenatal diagnosis at 10-24 weeks' gestation by a combination of chorionic villus sampling, amniocentesis, and fetal blood sampling. Polymerase chain reaction (PCR) was done on fetal and placental tissues, using primers which define a 221 bp region of the gene coding for the 44 kD protein of VZV. Positive cases were further analysed by dot blot hybridization, using radiolabelled DNA probes corresponding to the Hind III fragment VZV genome. The rate of placental/fetal infection was 36 per cent (5/14 fetuses: 2/11 in the first and 3/3 in the second trimester). At post-mortem examination, two aborted fetuses had hydrocephaly and VZV DNA was found in most of the examined tissues. The nine women who tested negative at prenatal investigation delivered healthy neonates whose VZV-specific IgM antibody titres were negative and none of them developed herpes tester infection. In view of the high frequency of fetal VZV infection and the reported low rate of malformations, the role of invasive prenatal diagnosis in women who acquire the infection in the first half of gestation is mainly that of reassurance when the test is negative.

AB - Fourteen fetuses at risk of Varicella-Zoster virus (VZV) infection underwent prenatal diagnosis at 10-24 weeks' gestation by a combination of chorionic villus sampling, amniocentesis, and fetal blood sampling. Polymerase chain reaction (PCR) was done on fetal and placental tissues, using primers which define a 221 bp region of the gene coding for the 44 kD protein of VZV. Positive cases were further analysed by dot blot hybridization, using radiolabelled DNA probes corresponding to the Hind III fragment VZV genome. The rate of placental/fetal infection was 36 per cent (5/14 fetuses: 2/11 in the first and 3/3 in the second trimester). At post-mortem examination, two aborted fetuses had hydrocephaly and VZV DNA was found in most of the examined tissues. The nine women who tested negative at prenatal investigation delivered healthy neonates whose VZV-specific IgM antibody titres were negative and none of them developed herpes tester infection. In view of the high frequency of fetal VZV infection and the reported low rate of malformations, the role of invasive prenatal diagnosis in women who acquire the infection in the first half of gestation is mainly that of reassurance when the test is negative.

KW - Polymerase chain reaction

KW - Prenatal diagnosis

KW - Varicella infection

UR - http://www.scopus.com/inward/record.url?scp=0030031034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030031034&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0223(199601)16:1<71::AID-PD806>3.0.CO;2-Z

DO - 10.1002/(SICI)1097-0223(199601)16:1<71::AID-PD806>3.0.CO;2-Z

M3 - Article

C2 - 8821856

AN - SCOPUS:0030031034

VL - 16

SP - 71

EP - 74

JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

SN - 0197-3851

IS - 1

ER -