Preoperative and intraoperative radioimmunodetection of cancer pretargeted by biotinylated monoclonal antibodies

Valerio Di Carlo, Paola De Nardi, Marco Stella, Patrizia Magnani, Ferruccio Fazio

Research output: Contribution to journalArticlepeer-review

Abstract

The in vivo post-targeting of tumor by means of anti-carcinoembryonic- antigen (CEA) monoclonal antibodies (MAbs) and the avidin-biotin three-step system was tested by immunoscintigraphy and radioimmunoguided surgery (RIGS) in six patients with primary or recurrent rectal cancer. The patients were preoperatively injected with 1 mg of FO23C5 (anti-CEA) and/or B72.3 anti- tumor-associated glycoprotein (TAG-72) biotinylated MAb; after 24 hours, 1 mg of avidin was administered, and, after a further 24 hours, biotin labeled to Indium-111 (In111) was injected. Preoperative imaging was obtained by means of a gamma camera, and a portable gamma-detecting probe (Neoprobe 1000) was intraoperatively used to count tumor and surrounding normal tissue. Eight tumor sites were localized in the six patients. Four lesions were identified preoperatively and six intraoperatively with a mean tumor-to-normal-tissue (T/NT) ratio of 1:8. This method allowed preoperative scintigraphy and intraoperative radioimmunodetection to be performed with a single radioactive compound injection of biotin labeled to In111 within few days before surgery.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalSeminars in Surgical Oncology
Volume15
Issue number4
DOIs
Publication statusPublished - Dec 1998

Keywords

  • Avidin
  • Biotin
  • Carcinoembryonic antigen
  • Colorectal neoplasms
  • DTPA
  • Gamma cameras
  • Indium radioisotopes
  • Intraoperative period
  • Iodine radioisotopes
  • Lymph node metastasis
  • Monoclonal antibodies
  • Radioimmunodetection
  • Rectal neoplasms

ASJC Scopus subject areas

  • Oncology
  • Surgery

Fingerprint Dive into the research topics of 'Preoperative and intraoperative radioimmunodetection of cancer pretargeted by biotinylated monoclonal antibodies'. Together they form a unique fingerprint.

Cite this