TY - JOUR
T1 - Preoperative and intraoperative radioimmunodetection of cancer pretargeted by biotinylated monoclonal antibodies
AU - Di Carlo, Valerio
AU - De Nardi, Paola
AU - Stella, Marco
AU - Magnani, Patrizia
AU - Fazio, Ferruccio
PY - 1998/12
Y1 - 1998/12
N2 - The in vivo post-targeting of tumor by means of anti-carcinoembryonic- antigen (CEA) monoclonal antibodies (MAbs) and the avidin-biotin three-step system was tested by immunoscintigraphy and radioimmunoguided surgery (RIGS) in six patients with primary or recurrent rectal cancer. The patients were preoperatively injected with 1 mg of FO23C5 (anti-CEA) and/or B72.3 anti- tumor-associated glycoprotein (TAG-72) biotinylated MAb; after 24 hours, 1 mg of avidin was administered, and, after a further 24 hours, biotin labeled to Indium-111 (In111) was injected. Preoperative imaging was obtained by means of a gamma camera, and a portable gamma-detecting probe (Neoprobe 1000) was intraoperatively used to count tumor and surrounding normal tissue. Eight tumor sites were localized in the six patients. Four lesions were identified preoperatively and six intraoperatively with a mean tumor-to-normal-tissue (T/NT) ratio of 1:8. This method allowed preoperative scintigraphy and intraoperative radioimmunodetection to be performed with a single radioactive compound injection of biotin labeled to In111 within few days before surgery.
AB - The in vivo post-targeting of tumor by means of anti-carcinoembryonic- antigen (CEA) monoclonal antibodies (MAbs) and the avidin-biotin three-step system was tested by immunoscintigraphy and radioimmunoguided surgery (RIGS) in six patients with primary or recurrent rectal cancer. The patients were preoperatively injected with 1 mg of FO23C5 (anti-CEA) and/or B72.3 anti- tumor-associated glycoprotein (TAG-72) biotinylated MAb; after 24 hours, 1 mg of avidin was administered, and, after a further 24 hours, biotin labeled to Indium-111 (In111) was injected. Preoperative imaging was obtained by means of a gamma camera, and a portable gamma-detecting probe (Neoprobe 1000) was intraoperatively used to count tumor and surrounding normal tissue. Eight tumor sites were localized in the six patients. Four lesions were identified preoperatively and six intraoperatively with a mean tumor-to-normal-tissue (T/NT) ratio of 1:8. This method allowed preoperative scintigraphy and intraoperative radioimmunodetection to be performed with a single radioactive compound injection of biotin labeled to In111 within few days before surgery.
KW - Avidin
KW - Biotin
KW - Carcinoembryonic antigen
KW - Colorectal neoplasms
KW - DTPA
KW - Gamma cameras
KW - Indium radioisotopes
KW - Intraoperative period
KW - Iodine radioisotopes
KW - Lymph node metastasis
KW - Monoclonal antibodies
KW - Radioimmunodetection
KW - Rectal neoplasms
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U2 - 10.1002/(SICI)1098-2388(199812)15:4<235::AID-SSU10>3.0.CO;2-B
DO - 10.1002/(SICI)1098-2388(199812)15:4<235::AID-SSU10>3.0.CO;2-B
M3 - Article
C2 - 9829379
AN - SCOPUS:0031789778
VL - 15
SP - 235
EP - 238
JO - Seminars in Surgical Oncology
JF - Seminars in Surgical Oncology
SN - 8756-0437
IS - 4
ER -