Prep1 prevents premature adipogenesis of mesenchymal progenitors

Giorgia Maroni, Vsevolod A. Tkachuk, Alexander Egorov, Marco J. Morelli, Raffaele Luongo, Elena Levantini, Francesco Blasi, Maria Cristina Magli, Dmitry Penkov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) in both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and in vitro 3T3-L1 preadipocytes significantly increases their adipogenic differentiation ability. Prep1 acts at a stage preceding the activation of the differentiation machinery because its DR makes cells more prone to adipogenic differentiation even in the absence of the adipogenic inducers. Prep1 DR expands the DNA binding landscape of C/EBPβ (CCAAT enhancer binding protein β) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPβ.

Original languageEnglish
Article number15573
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Adipogenesis
CCAAT-Enhancer-Binding Proteins
Down-Regulation
Transcription Factors
DNA
Mesenchymal Stromal Cells
Adipocytes
Bone Marrow

ASJC Scopus subject areas

  • General

Cite this

Maroni, G., Tkachuk, V. A., Egorov, A., Morelli, M. J., Luongo, R., Levantini, E., ... Penkov, D. (2017). Prep1 prevents premature adipogenesis of mesenchymal progenitors. Scientific Reports, 7(1), [15573]. https://doi.org/10.1038/s41598-017-15828-1

Prep1 prevents premature adipogenesis of mesenchymal progenitors. / Maroni, Giorgia; Tkachuk, Vsevolod A.; Egorov, Alexander; Morelli, Marco J.; Luongo, Raffaele; Levantini, Elena; Blasi, Francesco; Magli, Maria Cristina; Penkov, Dmitry.

In: Scientific Reports, Vol. 7, No. 1, 15573, 01.12.2017.

Research output: Contribution to journalArticle

Maroni, G, Tkachuk, VA, Egorov, A, Morelli, MJ, Luongo, R, Levantini, E, Blasi, F, Magli, MC & Penkov, D 2017, 'Prep1 prevents premature adipogenesis of mesenchymal progenitors', Scientific Reports, vol. 7, no. 1, 15573. https://doi.org/10.1038/s41598-017-15828-1
Maroni G, Tkachuk VA, Egorov A, Morelli MJ, Luongo R, Levantini E et al. Prep1 prevents premature adipogenesis of mesenchymal progenitors. Scientific Reports. 2017 Dec 1;7(1). 15573. https://doi.org/10.1038/s41598-017-15828-1
Maroni, Giorgia ; Tkachuk, Vsevolod A. ; Egorov, Alexander ; Morelli, Marco J. ; Luongo, Raffaele ; Levantini, Elena ; Blasi, Francesco ; Magli, Maria Cristina ; Penkov, Dmitry. / Prep1 prevents premature adipogenesis of mesenchymal progenitors. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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