Preparation and characterization of an intravenous solution of IgG from human immunodeficiency virus-seropositive donors

Larry M. Cummins, Kent J. Weinhold, Thomas J. Matthews, Alphonse J. Langlois, Carlo F. Perno, Richard M. Condie, Jean Pierre Allain

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Abstract

An intravenous solution of 99% pure globulin (hyperimmune IgG, HIVIG) was obtained from pooled plasma of selected human immunodeficiency virus (HIV-1)-seropositive asymptomatic donors with > 400 CD4+/μL cells per microliter and a high titer of antibody to HIV-1 p24 protein. HIVIG had high titers of antibody to p24, glycoprotein 41 (gp41), and gp120, group-specific neutralizing activity, and binding to the gp120 hypervariable loop region. It inhibited syncytia formation. At low concentration, it enhanced viral production of HIV-1 in infected peripheral blood monocytes but was inhibitory at higher concentration. HIVIG directed group-specific antibody-dependent cellular cytotoxicity against HIV-infected targets. For a period of 6 to 28 months, plasma donors kept stable antibody titers and had a 1.0% decrease in CD4+ cells per month. One gram per kilogram HIVIG injected in two juvenile chimpanzees was well tolerated and did not transmit HIV, as measured by negative cell culture, IgM immune response to HIV proteins, and polymerase chain reaction. The mean half-life of HIV-1 p24 antibody was 15 days. These preliminary data suggest that HIVIG is a safe product suitable for clinical trial in HIV-1-infected individuals.

Original languageEnglish
Pages (from-to)1111-1117
Number of pages7
JournalBlood
Volume77
Issue number5
Publication statusPublished - Mar 1 1991

ASJC Scopus subject areas

  • Hematology

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    Cummins, L. M., Weinhold, K. J., Matthews, T. J., Langlois, A. J., Perno, C. F., Condie, R. M., & Allain, J. P. (1991). Preparation and characterization of an intravenous solution of IgG from human immunodeficiency virus-seropositive donors. Blood, 77(5), 1111-1117.