Preparation of 4,4'-diaminodiphenylmethane-(2H4) for use as internal standard in the quantification of 4,4'-diaminodiphenylmethane

M. Natangelo; R. Bagnati; R. Fanelli; M. Gavinelli; C. Ulbricht; E. Benfenati

Preparation of 4,4'-diaminodiphenylmethane-(2H4) for use as internal standard in the quantification of 4,4'-diaminodiphenylmethane

M. Natangelo, R. Bagnati, R. Fanelli, M. Gavinelli, C. Ulbricht, E. Benfenati

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

A simple method has been developed for the synthesis of 4,4'-diaminodiphenylmethane-(²H₄) for analytical purposes in gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS). It consists in a proton-deuterium exchange reaction of the diamine using deutero-hydrochloric acid in deuterated methanol. The reaction was conducted at 60°C. Deuteration of the diamine in the ortho positions of the aromatic rings was observed after eight days in these conditions, using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.

Original language	English
Pages (from-to)	725-728
Number of pages	4
Journal	Journal of Labelled Compounds and Radiopharmaceuticals
Volume	29
Issue number	6
Publication status	Published - 1991

ASJC Scopus subject areas

Analytical Chemistry
Drug Discovery
Organic Chemistry
Clinical Biochemistry
Molecular Medicine
Pharmacology

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Preparation of 4,4'-diaminodiphenylmethane-(2H4) for use as internal standard in the quantification of 4,4'-diaminodiphenylmethane. / Natangelo, M.; Bagnati, R.; Fanelli, R.; Gavinelli, M.; Ulbricht, C.; Benfenati, E.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 29, No. 6, 1991, p. 725-728.

Research output: Contribution to journal › Article › peer-review

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AU - Gavinelli, M.

AU - Ulbricht, C.

AU - Benfenati, E.

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