Preparation of 4,4'-diaminodiphenylmethane-(2H4) for use as internal standard in the quantification of 4,4'-diaminodiphenylmethane

M. Natangelo, R. Bagnati, R. Fanelli, M. Gavinelli, C. Ulbricht, E. Benfenati

Research output: Contribution to journalArticle


A simple method has been developed for the synthesis of 4,4'-diaminodiphenylmethane-(2H4) for analytical purposes in gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS). It consists in a proton-deuterium exchange reaction of the diamine using deutero-hydrochloric acid in deuterated methanol. The reaction was conducted at 60°C. Deuteration of the diamine in the ortho positions of the aromatic rings was observed after eight days in these conditions, using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.

Original languageEnglish
Pages (from-to)725-728
Number of pages4
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number6
Publication statusPublished - 1991


ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Organic Chemistry
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

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