Preparation of a partially desialyated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

P. G. Crosignani, P. Donini, G. C. Lombroso

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the present of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.

Original languageEnglish
Pages (from-to)232-236
Number of pages5
JournalActa Endocrinologica
Volume95
Issue number2
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Preparation of a partially desialyated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin'. Together they form a unique fingerprint.

Cite this