Preparation of an antibody recognizing both human and rodent MRP1

Cristina Fernetti, Lorella Pascolo, Elena Podda, Renato Gennaro, Marco Stebel, Claudio Tiribelli

Research output: Contribution to journalArticle

Abstract

Based on the high level of identity among human, mouse, and rat MRP1 protein sequence, we produced a specific polyclonal antibody (MRP1-A23) against a synthetic polypeptide covering the C-terminus of the human protein. Western blot analysis showed a reactivity against human MRP1 similar to that obtained with the monoclonal QCRL1 antibody. Differently from other available antibodies against human MPR1, MRP1-A23 also detected both rat and mouse MRP1. No cross-reactivity was observed with either human or mouse MRP2 while MRP1-A23 weakly cross-reacted with rat MRP2 in the protein region ranging from 1512 to 1533 amino acids. These data indicate that MRP1-A23 allows specific MRP1 detection in both human and rodent tissues and may provide an important tool in the study of MRP1 expression and function in both experimental and clinical materials.

Original languageEnglish
Pages (from-to)1064-1068
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number4
DOIs
Publication statusPublished - Nov 9 2001

Keywords

  • Antibody
  • Human
  • Mouse
  • MRP1
  • Peptide
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Fernetti, C., Pascolo, L., Podda, E., Gennaro, R., Stebel, M., & Tiribelli, C. (2001). Preparation of an antibody recognizing both human and rodent MRP1. Biochemical and Biophysical Research Communications, 288(4), 1064-1068. https://doi.org/10.1006/bbrc.2001.5885