Preparation of colony stimulating activity from human leukemic urine: Hydrophobic chromatography on propylamine-agarose

R. Barresi, G. L B Scarra, R. Ravazzolo, M. Sessarego

Research output: Contribution to journalArticle

Abstract

Urinary colony stimulating factor (CSF) from human leukemic urine is retained on prophylamine-agarose hydrophibic chromatography; about 70% of the total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Incomplete binding of urinary CSF to this resin further confirms that biological activity is a heterogeneous pool of different molecular species. Analysis of the macro- and microscopic composition of colonies indicates that CSF resulting from this purification step maintains the ability to stimulate both granulocytic and macrophage progenitors.

Original languageEnglish
Pages (from-to)178-181
Number of pages4
JournalBiomedicine Express
Volume33
Issue number6
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Medicine(all)

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