Preparation of colony stimulating activity from human leukemic urine: Hydrophobic chromatography on propylamine-agarose

Urinary colony stimulating factor (CSF) from human leukemic urine is retained on prophylamine-agarose hydrophibic chromatography; about 70% of the total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Incomplete binding of urinary CSF to this resin further confirms that biological activity is a heterogeneous pool of different molecular species. Analysis of the macro- and microscopic composition of colonies indicates that CSF resulting from this purification step maintains the ability to stimulate both granulocytic and macrophage progenitors.