Preparation of lyso-GM1 (Il3Neu5AcGgOse4-long chain bases) by a one-pot reaction

S. Sonnino, D. Acquotti, G. Kirschner, A. Uguaglianza, L. Zecca, F. Rubino, G. Tettamanti

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A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one- pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90°C, 0.2 N KOH, 1 mM GM1) was 54%. The chemical structure of lyso-GM1 was determined by 1H-NMR and FAB-MS analyses, thus proving that the acetamide groups of galactosamine and sialic acid units were not affected during the deacylation reaction.

Original languageEnglish
Pages (from-to)1221-1226
Number of pages6
JournalJournal of Lipid Research
Issue number8
Publication statusPublished - 1992


  • gangliosides
  • GM1
  • lyso-gangliosides

ASJC Scopus subject areas

  • Endocrinology


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