Präparation von Kaninchen-Antikörpern gegen 4,4'-Dimethoxytriphenylmethyl, der in der Oligonudeotidsynthese verwendeten Schutzgruppe

Translated title of the contribution: Preparation of Rabbit Antibodies to 4,4'-Dimethoxytriphenylmethyl, The Protective Group in Oligonucleotide Synthesis

Alberto Chersi, Teresa Francesca Romano, Michele Evangelista, Anna Maria Mileo, Giuliana Falasca

Research output: Contribution to journalArticle

Abstract

Antibodies were raised in 2 rabbits by immunization with carrier proteins covalently bound to deoxyguanosine bearing a 4,4'-dimethoxytriphenylmethyl group protecting the 5'-hydroxy terminus of deoxyribose. After several injections with such complexes, immune sera were tested with an immuno-enzymatic method using as antigens several compounds containing the hapten, as well as synthetic oligonucleotides bearing, or not, this protective group at the 5' terminus. One of the two antisera appeared to recognize the dimethoxytrityl group bound to carrier molecules, and thus might find a useful application for the detection, quantitation, and control of oligonucleotides obtained by automatic synthesis.

Original languageUndefined/Unknown
Pages (from-to)845-848
Number of pages4
JournalBiological Chemistry Hoppe-Seyler
Volume372
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Bearings (structural)
Oligonucleotides
Immune Sera
Rabbits
Deoxyribose
Immunization
Deoxyguanosine
Antibodies
Haptens
Carrier Proteins
Antigens
Injections
Molecules
4,4'-dimethoxytrityl cation

Keywords

  • DMT
  • ELISA
  • Oligonucleotides
  • rabbit antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Präparation von Kaninchen-Antikörpern gegen 4,4'-Dimethoxytriphenylmethyl, der in der Oligonudeotidsynthese verwendeten Schutzgruppe. / Chersi, Alberto; Romano, Teresa Francesca; Evangelista, Michele; Mileo, Anna Maria; Falasca, Giuliana.

In: Biological Chemistry Hoppe-Seyler, Vol. 372, No. 2, 1991, p. 845-848.

Research output: Contribution to journalArticle

Chersi, Alberto ; Romano, Teresa Francesca ; Evangelista, Michele ; Mileo, Anna Maria ; Falasca, Giuliana. / Präparation von Kaninchen-Antikörpern gegen 4,4'-Dimethoxytriphenylmethyl, der in der Oligonudeotidsynthese verwendeten Schutzgruppe. In: Biological Chemistry Hoppe-Seyler. 1991 ; Vol. 372, No. 2. pp. 845-848.
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