Preparation of the tritiated molecular forms of gangliosides with homogeneous long chain base composition

Guiliano Gazzotti, Sandro Sonnino, Riccardo Ghidoni, Paolo Orlando, Guido Tettamanti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Gangliosides GM2, GM1 and GD1b were radiolabelled at C-6 of the terminal galactose or N-acetylgalactosamine by the galactose oxidase/[3H]NaBH4 method; gangliosides GM2, GM1, Fuc-GM1 and GD1a were radiolabelled at C-3 of the long chain base by the 2,3-dichloro-5,6-dicyanobenzoquinone/[3H]NaBH4 method. By application of an original HPLC procedure, eight different molecular species were prepared from each labelled ganglioside. Each of these species was characterized by the presence of one of the following long chain bases:erythro C18 sphingosine, threo C18 sphingosine, erythro C18 sphinganine, threo C18 sphinganine, erythro C20 sphingosine, threo C20 sphingosine, erythro C20 sphinganine and threo C20 sphinganine. From GD1b only the species containing the erythro forms of long chain bases were obtained. The individual molecular species were more than 99% homogeneous and had a radiopurity better than 99%. The molecular species of the same ganglioside, radiolabelled at C-3 of the long chain base, had identical specific radioactivity, namely 1.17, 1.25, 0.85 and 1.28 Ci/mmol for GM2, GM1, Fuc-GM1 and GD1a respectively. The molecular species of the same ganglioside, radiolabelled at C-6 of terminal galactose or N-acetylgalactosamine, had similar specific radioactivity, namely 1.34-1.40, 1.44-1.51, 1.37-1.44 Ci/mmol for GM2, GM1 and GD1b respectively.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalGlycoconjugate Journal
Volume1
Issue number2
DOIs
Publication statusPublished - Sep 1984

Fingerprint

Gangliosides
Base Composition
G(M2) Ganglioside
G(M1) Ganglioside
Acetylgalactosamine
Sphingosine
Radioactivity
Chemical analysis
Galactose
Galactose Oxidase
High Pressure Liquid Chromatography
erythro-(2R,3S)-sphingosine
safingol

Keywords

  • ganglioside long chain bases
  • ganglioside radioactive labelling
  • HPLC separation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Preparation of the tritiated molecular forms of gangliosides with homogeneous long chain base composition. / Gazzotti, Guiliano; Sonnino, Sandro; Ghidoni, Riccardo; Orlando, Paolo; Tettamanti, Guido.

In: Glycoconjugate Journal, Vol. 1, No. 2, 09.1984, p. 111-121.

Research output: Contribution to journalArticle

Gazzotti, Guiliano ; Sonnino, Sandro ; Ghidoni, Riccardo ; Orlando, Paolo ; Tettamanti, Guido. / Preparation of the tritiated molecular forms of gangliosides with homogeneous long chain base composition. In: Glycoconjugate Journal. 1984 ; Vol. 1, No. 2. pp. 111-121.
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abstract = "Gangliosides GM2, GM1 and GD1b were radiolabelled at C-6 of the terminal galactose or N-acetylgalactosamine by the galactose oxidase/[3H]NaBH4 method; gangliosides GM2, GM1, Fuc-GM1 and GD1a were radiolabelled at C-3 of the long chain base by the 2,3-dichloro-5,6-dicyanobenzoquinone/[3H]NaBH4 method. By application of an original HPLC procedure, eight different molecular species were prepared from each labelled ganglioside. Each of these species was characterized by the presence of one of the following long chain bases:erythro C18 sphingosine, threo C18 sphingosine, erythro C18 sphinganine, threo C18 sphinganine, erythro C20 sphingosine, threo C20 sphingosine, erythro C20 sphinganine and threo C20 sphinganine. From GD1b only the species containing the erythro forms of long chain bases were obtained. The individual molecular species were more than 99{\%} homogeneous and had a radiopurity better than 99{\%}. The molecular species of the same ganglioside, radiolabelled at C-3 of the long chain base, had identical specific radioactivity, namely 1.17, 1.25, 0.85 and 1.28 Ci/mmol for GM2, GM1, Fuc-GM1 and GD1a respectively. The molecular species of the same ganglioside, radiolabelled at C-6 of terminal galactose or N-acetylgalactosamine, had similar specific radioactivity, namely 1.34-1.40, 1.44-1.51, 1.37-1.44 Ci/mmol for GM2, GM1 and GD1b respectively.",
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AB - Gangliosides GM2, GM1 and GD1b were radiolabelled at C-6 of the terminal galactose or N-acetylgalactosamine by the galactose oxidase/[3H]NaBH4 method; gangliosides GM2, GM1, Fuc-GM1 and GD1a were radiolabelled at C-3 of the long chain base by the 2,3-dichloro-5,6-dicyanobenzoquinone/[3H]NaBH4 method. By application of an original HPLC procedure, eight different molecular species were prepared from each labelled ganglioside. Each of these species was characterized by the presence of one of the following long chain bases:erythro C18 sphingosine, threo C18 sphingosine, erythro C18 sphinganine, threo C18 sphinganine, erythro C20 sphingosine, threo C20 sphingosine, erythro C20 sphinganine and threo C20 sphinganine. From GD1b only the species containing the erythro forms of long chain bases were obtained. The individual molecular species were more than 99% homogeneous and had a radiopurity better than 99%. The molecular species of the same ganglioside, radiolabelled at C-3 of the long chain base, had identical specific radioactivity, namely 1.17, 1.25, 0.85 and 1.28 Ci/mmol for GM2, GM1, Fuc-GM1 and GD1a respectively. The molecular species of the same ganglioside, radiolabelled at C-6 of terminal galactose or N-acetylgalactosamine, had similar specific radioactivity, namely 1.34-1.40, 1.44-1.51, 1.37-1.44 Ci/mmol for GM2, GM1 and GD1b respectively.

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