Presence and characterization of the dopamine transporter in human resting lymphocytes

Donatella Marazziti; Stefano Baroni; Mario Catena Dell'Osso; Irene Masala; Laura Fabbrini; Laura Betti; Gino Giannaccini; Bernardo Dell'Osso; Antonio Lucacchini

doi:10.1007/s11064-007-9541-4

Presence and characterization of the dopamine transporter in human resting lymphocytes

Donatella Marazziti, Stefano Baroni, Mario Catena Dell'Osso, Irene Masala, Laura Fabbrini, Laura Betti, Gino Giannaccini, Bernardo Dell'Osso, Antonio Lucacchini

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of ³H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of ³H-dopamine (³H-DA). Lymphocytes were obtained by 15 healthy subjects. The results showed the presence of a specific and saturable binding of ³H-WIN 35,428, which labelled one site only. A specific ³H-DA reuptake was also measured. The pharmacological characterization of both binding and reuptake was overlapping. These findings would indicate that human resting lymphocytes carry the DAT, whose functions in periphery are still unknown.

Original language	English
Pages (from-to)	1011-1016
Number of pages	6
Journal	Neurochemical Research
Volume	33
Issue number	6
DOIs	https://doi.org/10.1007/s11064-007-9541-4
Publication status	Published - Jun 2008

Keywords

H-DA reuptake
H-WIN-35,428 binding
Dopamine
Dopamine transporter
Human lymphocytes
Pharmacological characterization

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry

Access to Document

10.1007/s11064-007-9541-4

Fingerprint Dive into the research topics of 'Presence and characterization of the dopamine transporter in human resting lymphocytes'. Together they form a unique fingerprint.

Cite this

Presence and characterization of the dopamine transporter in human resting lymphocytes. / Marazziti, Donatella; Baroni, Stefano; Catena Dell'Osso, Mario; Masala, Irene; Fabbrini, Laura; Betti, Laura; Giannaccini, Gino; Dell'Osso, Bernardo; Lucacchini, Antonio.

In: Neurochemical Research, Vol. 33, No. 6, 06.2008, p. 1011-1016.

Research output: Contribution to journal › Article › peer-review

@article{8bf75b9f5cc248fbbe65813d69e26371,

title = "Presence and characterization of the dopamine transporter in human resting lymphocytes",

abstract = "The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Lymphocytes were obtained by 15 healthy subjects. The results showed the presence of a specific and saturable binding of 3H-WIN 35,428, which labelled one site only. A specific 3H-DA reuptake was also measured. The pharmacological characterization of both binding and reuptake was overlapping. These findings would indicate that human resting lymphocytes carry the DAT, whose functions in periphery are still unknown.",

keywords = "H-DA reuptake, H-WIN-35,428 binding, Dopamine, Dopamine transporter, Human lymphocytes, Pharmacological characterization",

author = "Donatella Marazziti and Stefano Baroni and {Catena Dell'Osso}, Mario and Irene Masala and Laura Fabbrini and Laura Betti and Gino Giannaccini and Bernardo Dell'Osso and Antonio Lucacchini",

year = "2008",

month = jun,

doi = "10.1007/s11064-007-9541-4",

language = "English",

volume = "33",

pages = "1011--1016",

journal = "Neurochemical Research",

issn = "0364-3190",

publisher = "Springer New York",

number = "6",

}

TY - JOUR

T1 - Presence and characterization of the dopamine transporter in human resting lymphocytes

AU - Marazziti, Donatella

AU - Baroni, Stefano

AU - Catena Dell'Osso, Mario

AU - Masala, Irene

AU - Fabbrini, Laura

AU - Betti, Laura

AU - Giannaccini, Gino

AU - Dell'Osso, Bernardo

AU - Lucacchini, Antonio

PY - 2008/6

Y1 - 2008/6

N2 - The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Lymphocytes were obtained by 15 healthy subjects. The results showed the presence of a specific and saturable binding of 3H-WIN 35,428, which labelled one site only. A specific 3H-DA reuptake was also measured. The pharmacological characterization of both binding and reuptake was overlapping. These findings would indicate that human resting lymphocytes carry the DAT, whose functions in periphery are still unknown.

AB - The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Lymphocytes were obtained by 15 healthy subjects. The results showed the presence of a specific and saturable binding of 3H-WIN 35,428, which labelled one site only. A specific 3H-DA reuptake was also measured. The pharmacological characterization of both binding and reuptake was overlapping. These findings would indicate that human resting lymphocytes carry the DAT, whose functions in periphery are still unknown.

KW - H-DA reuptake

KW - H-WIN-35,428 binding

KW - Dopamine

KW - Dopamine transporter

KW - Human lymphocytes

KW - Pharmacological characterization

UR - http://www.scopus.com/inward/record.url?scp=42149133661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149133661&partnerID=8YFLogxK

U2 - 10.1007/s11064-007-9541-4

DO - 10.1007/s11064-007-9541-4

M3 - Article

C2 - 18038207

AN - SCOPUS:42149133661

VL - 33

SP - 1011

EP - 1016

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 6

ER -