Abstract
We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 397-409 |
| Number of pages | 13 |
| Journal | BBA - General Subjects |
| Volume | 1474 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1 2000 |
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Keywords
- Apoptosis
- Catalase cytochemistry
- Neuroectodermal cell line
- Perfluorodecanoic acid
- Peroxisome proliferator
- Peroxisome proliferator-activated receptor
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Presence and inducibility of peroxisomes in a human glioblastoma cell line. / Cimini, A.; Cristiano, L.; Bernardo, A.; Farioli-Vecchioli, S.; Stefanini, S.; Cerù, M. P.
In: BBA - General Subjects, Vol. 1474, No. 3, 01.05.2000, p. 397-409.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence and inducibility of peroxisomes in a human glioblastoma cell line
AU - Cimini, A.
AU - Cristiano, L.
AU - Bernardo, A.
AU - Farioli-Vecchioli, S.
AU - Stefanini, S.
AU - Cerù, M. P.
PY - 2000/5/1
Y1 - 2000/5/1
N2 - We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.
AB - We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.
KW - Apoptosis
KW - Catalase cytochemistry
KW - Neuroectodermal cell line
KW - Perfluorodecanoic acid
KW - Peroxisome proliferator
KW - Peroxisome proliferator-activated receptor
UR - http://www.scopus.com/inward/record.url?scp=0034193717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034193717&partnerID=8YFLogxK
U2 - 10.1016/S0304-4165(00)00036-2
DO - 10.1016/S0304-4165(00)00036-2
M3 - Article
C2 - 10779693
AN - SCOPUS:0034193717
VL - 1474
SP - 397
EP - 409
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -