Presence and inducibility of peroxisomes in a human glioblastoma cell line

A. Cimini, L. Cristiano, A. Bernardo, S. Farioli-Vecchioli, S. Stefanini, M. P. Cerù

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)397-409
Number of pages13
JournalBBA - General Subjects
Volume1474
Issue number3
DOIs
Publication statusPublished - May 1 2000

Fingerprint

Peroxisomes
Glioblastoma
Peroxisome Proliferator-Activated Receptors
Cells
Cell Line
Cell proliferation
Cell Proliferation
Enzyme activity
Catalase
perfluorodecanoic acid
Proteins
Chemical activation
Association reactions
Apoptosis
Enzymes

Keywords

  • Apoptosis
  • Catalase cytochemistry
  • Neuroectodermal cell line
  • Perfluorodecanoic acid
  • Peroxisome proliferator
  • Peroxisome proliferator-activated receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cimini, A., Cristiano, L., Bernardo, A., Farioli-Vecchioli, S., Stefanini, S., & Cerù, M. P. (2000). Presence and inducibility of peroxisomes in a human glioblastoma cell line. BBA - General Subjects, 1474(3), 397-409. https://doi.org/10.1016/S0304-4165(00)00036-2

Presence and inducibility of peroxisomes in a human glioblastoma cell line. / Cimini, A.; Cristiano, L.; Bernardo, A.; Farioli-Vecchioli, S.; Stefanini, S.; Cerù, M. P.

In: BBA - General Subjects, Vol. 1474, No. 3, 01.05.2000, p. 397-409.

Research output: Contribution to journalArticle

Cimini, A, Cristiano, L, Bernardo, A, Farioli-Vecchioli, S, Stefanini, S & Cerù, MP 2000, 'Presence and inducibility of peroxisomes in a human glioblastoma cell line', BBA - General Subjects, vol. 1474, no. 3, pp. 397-409. https://doi.org/10.1016/S0304-4165(00)00036-2
Cimini A, Cristiano L, Bernardo A, Farioli-Vecchioli S, Stefanini S, Cerù MP. Presence and inducibility of peroxisomes in a human glioblastoma cell line. BBA - General Subjects. 2000 May 1;1474(3):397-409. https://doi.org/10.1016/S0304-4165(00)00036-2
Cimini, A. ; Cristiano, L. ; Bernardo, A. ; Farioli-Vecchioli, S. ; Stefanini, S. ; Cerù, M. P. / Presence and inducibility of peroxisomes in a human glioblastoma cell line. In: BBA - General Subjects. 2000 ; Vol. 1474, No. 3. pp. 397-409.
@article{7df0e8ced0fe4e42a79df1cb488ba370,
title = "Presence and inducibility of peroxisomes in a human glioblastoma cell line",
abstract = "We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Apoptosis, Catalase cytochemistry, Neuroectodermal cell line, Perfluorodecanoic acid, Peroxisome proliferator, Peroxisome proliferator-activated receptor",
author = "A. Cimini and L. Cristiano and A. Bernardo and S. Farioli-Vecchioli and S. Stefanini and Cer{\`u}, {M. P.}",
year = "2000",
month = "5",
day = "1",
doi = "10.1016/S0304-4165(00)00036-2",
language = "English",
volume = "1474",
pages = "397--409",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Presence and inducibility of peroxisomes in a human glioblastoma cell line

AU - Cimini, A.

AU - Cristiano, L.

AU - Bernardo, A.

AU - Farioli-Vecchioli, S.

AU - Stefanini, S.

AU - Cerù, M. P.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.

AB - We investigated the effect of the peroxisomal proliferator (PP) perfluorodecanoic acid (PFDA), alone or in combination with 9-cis-retinoic acid (RX) on the human glioblastoma cell line Lipari (LI). Cell proliferation, apoptotic rate, peroxisome morphology and morphometry, peroxisomal enzyme activities and the presence of peroxisome proliferator-activated receptors (PPARs) were examined. We show that PFDA alone produces pleiotropic effects on LI cells and that RX enhances some of these effects. Peroxisomal number and relative volume, as well as palmitoyl-CoA oxidase activity and protein, are increased by PFDA treatment, with a synergistic effect by RX. The latter, alone or in association with PFDA, induces catalase activity and protein, increases apoptosis and decreases cell proliferation. PPAR isotypes α and γ were detected in LI cells. While the former is apparently unaffected by either treatment, the latter increases in response to PFDA, independent of the presence of RX. The results of this study are discussed in terms of PPARα activation and PPARγ induction by PFDA, by either a direct or an indirect mechanism. Copyright (C) 2000 Elsevier Science B.V.

KW - Apoptosis

KW - Catalase cytochemistry

KW - Neuroectodermal cell line

KW - Perfluorodecanoic acid

KW - Peroxisome proliferator

KW - Peroxisome proliferator-activated receptor

UR - http://www.scopus.com/inward/record.url?scp=0034193717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034193717&partnerID=8YFLogxK

U2 - 10.1016/S0304-4165(00)00036-2

DO - 10.1016/S0304-4165(00)00036-2

M3 - Article

VL - 1474

SP - 397

EP - 409

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 3

ER -