Presence of di- and polyamines covalently bound to protein in rat liver

Simone Beninati, Mauro Piacentini, Maria Paola Argento-Cerù, Salvatore Russo-Caia, Francesco Autuori

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Acid hydrolysis of trichloroacetic acid precipitate from rat tissue (liver, kidney and testis) homogenate released significant amounts of acid-insoluble putrescine, spermidine and spermine. Following incubation of liver homogenate with [1,4-14C]putrescine, 1.4% of total radioactivity and 1.0% of labelled diamine were recovered in the acid-insoluble fraction. Exhaustive digestion of acid-precipitable material with proteinases (Pronase, aminopeptidase M, carboxipeptidase A, B and Y) revealed the presence of di- and polyamines and of N1-(γ-glutamyl)spermidine, N1-(γ-glutamyl)sperminine and N1, N12-bis(γ-glutamyl)spermine. These derivatives were identified both by chromatographic analysis and by enzymatic digestion with purified γ-glutamylamine cyclotransferase. The finding of di- and polyamine γ-glutamyl derivatives in the proteinase-digested acid-insoluble fraction of homogenate may be considered as a proof of the in vivo transglutaminase-catalyzed binding of polyamines to proteins. This evidence suggests that di- and polyamines might have an important role in mammalian tissues through covalent binding to proteins by either one or both the primary amino groups.

Original languageEnglish
Pages (from-to)120-126
Number of pages7
JournalBBA - General Subjects
Volume841
Issue number1
DOIs
Publication statusPublished - Jul 26 1985

Fingerprint

Diamines
Polyamines
Liver
Rats
Acids
Putrescine
Spermidine
Spermine
Proteins
Digestion
Peptide Hydrolases
Chromatographic analysis
Tissue
Derivatives
CD13 Antigens
Trichloroacetic Acid
Pronase
Transglutaminases
Radioactivity
Testis

Keywords

  • (Rat liver)
  • Polyamine γ-glutamyl derivative
  • Polyamine-protein complexe

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Presence of di- and polyamines covalently bound to protein in rat liver. / Beninati, Simone; Piacentini, Mauro; Argento-Cerù, Maria Paola; Russo-Caia, Salvatore; Autuori, Francesco.

In: BBA - General Subjects, Vol. 841, No. 1, 26.07.1985, p. 120-126.

Research output: Contribution to journalArticle

Beninati, Simone ; Piacentini, Mauro ; Argento-Cerù, Maria Paola ; Russo-Caia, Salvatore ; Autuori, Francesco. / Presence of di- and polyamines covalently bound to protein in rat liver. In: BBA - General Subjects. 1985 ; Vol. 841, No. 1. pp. 120-126.
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