Presence of fetal DNA in maternal plasma decades after pregnancy

Pietro Invernizzi, Maria Luisa Biondi, Pier Maria Battezzati, Francesca Perego, Carlo Selmi, Federica Cecchini, Mauro Podda, Giuseppe Simoni

Research output: Contribution to journalArticlepeer-review

Abstract

Cells of fetal origin and cell-free fetal DNA can be detected in the maternal circulation during pregnancy, and it has recently been shown that fetal cells can persist long after delivery. Given the various biological and clinical implications of this fact, we tested the hypothesis that cell-free fetal DNA can be present in maternal plasma decades after pregnancy. We extracted DNA from plasma samples and nucleated blood cells of 160 healthy women with male offspring at different time intervals after delivery (range 1-60 years). All of the samples were tested by means of a real-time quantitative PCR assay for a specific Y chromosome sequence (the SRY gene). Y chromosome-specific DNA was detected in 16 peripheral blood cell samples (10%) and 35 plasma samples (22%). The women with male sequences in the cell fraction had significantly greater total parity (P=0.018). The proportion of women with detectable Y sequences in the plasma or cell samples was not related to the time since delivery. The fetal DNA concentrations in the genomic material extracted from plasma samples were significantly higher than those extracted from the Y-positive cell samples (149±140 vs 20±13 genome-equivalents/ml; P

Original languageEnglish
Pages (from-to)587-591
Number of pages5
JournalHuman Genetics
Volume110
Issue number6
DOIs
Publication statusPublished - Jun 2002

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

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