Presence of heterogeneous peroxisomal populations in the rat nervous tissue

A. M. Cimini, I. Singh, S. Farioli-Vecchioli, L. Cristiano, M. P. Cerú

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxisomes were purified from the nervous tissue of 14-day-old rats by means of a Nycodenz gradient. Peroxisomal enzymes exhibited different sedimentation patterns: dihydroxyacetone phosphate acyl-transferase equilibrates at 1.142 g/ml together with the first peak of catalase; palmitoyl-CoA oxidase and d-amino acid oxidase activities are mainly recovered at 1.154 g/ml; the second peak of catalase is found at 1.175 g/ml. Morphological and semi-quantitative analyses of immunogold-labelled peroxisomes reveal profound heterogeneity of the particles. Very small (=0.2 μm diameter), electron dense vesicles containing catalase or thiolase, but devoid of other tested enzymes, are preferentially found in the light region, together with larger (>0.2

Original languageEnglish
Pages (from-to)13-26
Number of pages14
JournalBBA - General Subjects
Volume1425
Issue number1
DOIs
Publication statusPublished - Sep 16 1998

Keywords

  • Brain
  • Central nervous system
  • Glia
  • Peroxisomal biogenesis
  • Peroxisomal disorder
  • Peroxisome
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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