Presence of L701 M mutation in the FKS1 gene of echinocandin-susceptible Candida krusei isolates

Fabiola Lallitto, Anna Prigitano, Francesca Mangione, Antonio Piralla, Francesca Tamarozzi, Piero Marone, Caterina Cavanna

Research output: Contribution to journalArticle

Abstract

Resistance of Candida krusei isolates to echinocandins, the recommended drugs to treat candidemia, has been associated with mutations in hot spot (HS) regions of the FKS1 gene or L701 M mutation in a region between HS1 and HS3 of FKS1. However, the role of L701 M mutation alone in causing reduced echinocandins susceptibility is still unclear. We analyzed a region between HS1 and HS3 of FKS1 of 25 C. krusei isolates from clinical samples. Susceptibility to echinocandins was determined by a commercial broth microdilution assay and by the microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The L701 M mutation was detected in 22/25 (88%) C. krusei isolates with low MIC values in the absence of other HS mutations. The presence of isolated L701 M mutation in C. krusei clinical isolates susceptible to echinocandins suggests that this mutation may be just associated to polymorphism in the C. krusei population.

Original languageEnglish
JournalDiagnostic Microbiology and Infectious Disease
DOIs
Publication statusAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Echinocandins
Candida
Mutation
Genes
Candidemia

Keywords

  • Candida krusei
  • Candidemia
  • Echinocandins
  • FKS1 gene

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Presence of L701 M mutation in the FKS1 gene of echinocandin-susceptible Candida krusei isolates",
abstract = "Resistance of Candida krusei isolates to echinocandins, the recommended drugs to treat candidemia, has been associated with mutations in hot spot (HS) regions of the FKS1 gene or L701 M mutation in a region between HS1 and HS3 of FKS1. However, the role of L701 M mutation alone in causing reduced echinocandins susceptibility is still unclear. We analyzed a region between HS1 and HS3 of FKS1 of 25 C. krusei isolates from clinical samples. Susceptibility to echinocandins was determined by a commercial broth microdilution assay and by the microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The L701 M mutation was detected in 22/25 (88{\%}) C. krusei isolates with low MIC values in the absence of other HS mutations. The presence of isolated L701 M mutation in C. krusei clinical isolates susceptible to echinocandins suggests that this mutation may be just associated to polymorphism in the C. krusei population.",
keywords = "Candida krusei, Candidemia, Echinocandins, FKS1 gene",
author = "Fabiola Lallitto and Anna Prigitano and Francesca Mangione and Antonio Piralla and Francesca Tamarozzi and Piero Marone and Caterina Cavanna",
year = "2018",
month = "1",
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doi = "10.1016/j.diagmicrobio.2018.07.005",
language = "English",
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TY - JOUR

T1 - Presence of L701 M mutation in the FKS1 gene of echinocandin-susceptible Candida krusei isolates

AU - Lallitto, Fabiola

AU - Prigitano, Anna

AU - Mangione, Francesca

AU - Piralla, Antonio

AU - Tamarozzi, Francesca

AU - Marone, Piero

AU - Cavanna, Caterina

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Resistance of Candida krusei isolates to echinocandins, the recommended drugs to treat candidemia, has been associated with mutations in hot spot (HS) regions of the FKS1 gene or L701 M mutation in a region between HS1 and HS3 of FKS1. However, the role of L701 M mutation alone in causing reduced echinocandins susceptibility is still unclear. We analyzed a region between HS1 and HS3 of FKS1 of 25 C. krusei isolates from clinical samples. Susceptibility to echinocandins was determined by a commercial broth microdilution assay and by the microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The L701 M mutation was detected in 22/25 (88%) C. krusei isolates with low MIC values in the absence of other HS mutations. The presence of isolated L701 M mutation in C. krusei clinical isolates susceptible to echinocandins suggests that this mutation may be just associated to polymorphism in the C. krusei population.

AB - Resistance of Candida krusei isolates to echinocandins, the recommended drugs to treat candidemia, has been associated with mutations in hot spot (HS) regions of the FKS1 gene or L701 M mutation in a region between HS1 and HS3 of FKS1. However, the role of L701 M mutation alone in causing reduced echinocandins susceptibility is still unclear. We analyzed a region between HS1 and HS3 of FKS1 of 25 C. krusei isolates from clinical samples. Susceptibility to echinocandins was determined by a commercial broth microdilution assay and by the microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The L701 M mutation was detected in 22/25 (88%) C. krusei isolates with low MIC values in the absence of other HS mutations. The presence of isolated L701 M mutation in C. krusei clinical isolates susceptible to echinocandins suggests that this mutation may be just associated to polymorphism in the C. krusei population.

KW - Candida krusei

KW - Candidemia

KW - Echinocandins

KW - FKS1 gene

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