Presence of multiple copies of the capsulation b locus in invasive Haemophilus influenzae type b (Hib) strains isolated from children with Hib conjugate vaccine failure

Marina Cerquetti, Rita Cardines, Marta Luisa Ciofi Degli Atti, Maria Giufré, Antonino Bella, Tonino Sofia, Paola Mastrantonio, Mary Slack

Research output: Contribution to journalArticle

Abstract

Most invasive Haemophilus influenzae type b strains possess a duplication of the capsulation locus. Further amplification resulting in as many as 5 copies has been described. To verify whether amplification is involved in vaccine failure, the number of copies of the locus was determined by Southern blotting in 90 strains from children with true vaccine failure (TVF) between 1993 and 1999 and in 139 strains from unvaccinated children (50 collected between 1993 and 1999 and 89 collected between 1991 and 1992, before routine immunization was introduced). A significantly greater proportion of strains from TVFs contained multiple copies, compared with strains from control children (24% vs. 10%; P = .0379), which suggests that amplification of the capb locus may be a contributory factor in vaccine failure. The presence of multiple-copy strains was associated with disease other than meningitis.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalJournal of Infectious Diseases
Volume192
Issue number5
DOIs
Publication statusPublished - Sep 1 2005

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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