Abstract
The product of the RET proto-oncogene is a protein belonging to the receptor-like tyrosine kinase superfamily. RET is expressed in several neural crest-derived cell lineages and has been implicated in the correct development of the peripheral nervous system. To gain further insight into RET function, we investigated the presence of active RET in adult rat tissues. We show, by immunoblotting, that the products of the RET proto- oncogene (p155(ret)) are present in specific regions of adult rat brain, including the cerebellum, striatum, brainstem, hypothalamus, hippocampus, and olfactory bulb. Moreover, in the cerebellum, p155(ret) is phosphorylated in tyrosine residues, thus indicating that this brain structure contains p155(ret) in an activated state. Finally, the presence of RET in motoneurons prompted us to analyze the effects of hypoglossal nerve section on its expression. We observed a dramatic increase in p155(ret) in the motoneuron nuclei, thus suggesting that RET tyrosine kinase plays a role in the neuronal response to axotomy and/or during nerve regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 1081-1086 |
| Number of pages | 6 |
| Journal | Cell Growth and Differentiation |
| Volume | 7 |
| Issue number | 8 |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Presence of physiologically stimulated RET in adult rat brain : Induction of RET expression during nerve regeneration. / Luca Colucci-D'Amato, G.; D'Alessio, Amelia; Filliatreau, Ghislaine; Florio, Tullio; Di Giamberardino, Luigi; Chiappetta, Gennaro; Vecchio, Giancarlo; Fusco, Alfredo; Santoro, Massimo; De Franciscis, Vittorio.
In: Cell Growth and Differentiation, Vol. 7, No. 8, 1996, p. 1081-1086.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence of physiologically stimulated RET in adult rat brain
T2 - Induction of RET expression during nerve regeneration
AU - Luca Colucci-D'Amato, G.
AU - D'Alessio, Amelia
AU - Filliatreau, Ghislaine
AU - Florio, Tullio
AU - Di Giamberardino, Luigi
AU - Chiappetta, Gennaro
AU - Vecchio, Giancarlo
AU - Fusco, Alfredo
AU - Santoro, Massimo
AU - De Franciscis, Vittorio
PY - 1996
Y1 - 1996
N2 - The product of the RET proto-oncogene is a protein belonging to the receptor-like tyrosine kinase superfamily. RET is expressed in several neural crest-derived cell lineages and has been implicated in the correct development of the peripheral nervous system. To gain further insight into RET function, we investigated the presence of active RET in adult rat tissues. We show, by immunoblotting, that the products of the RET proto- oncogene (p155(ret)) are present in specific regions of adult rat brain, including the cerebellum, striatum, brainstem, hypothalamus, hippocampus, and olfactory bulb. Moreover, in the cerebellum, p155(ret) is phosphorylated in tyrosine residues, thus indicating that this brain structure contains p155(ret) in an activated state. Finally, the presence of RET in motoneurons prompted us to analyze the effects of hypoglossal nerve section on its expression. We observed a dramatic increase in p155(ret) in the motoneuron nuclei, thus suggesting that RET tyrosine kinase plays a role in the neuronal response to axotomy and/or during nerve regeneration.
AB - The product of the RET proto-oncogene is a protein belonging to the receptor-like tyrosine kinase superfamily. RET is expressed in several neural crest-derived cell lineages and has been implicated in the correct development of the peripheral nervous system. To gain further insight into RET function, we investigated the presence of active RET in adult rat tissues. We show, by immunoblotting, that the products of the RET proto- oncogene (p155(ret)) are present in specific regions of adult rat brain, including the cerebellum, striatum, brainstem, hypothalamus, hippocampus, and olfactory bulb. Moreover, in the cerebellum, p155(ret) is phosphorylated in tyrosine residues, thus indicating that this brain structure contains p155(ret) in an activated state. Finally, the presence of RET in motoneurons prompted us to analyze the effects of hypoglossal nerve section on its expression. We observed a dramatic increase in p155(ret) in the motoneuron nuclei, thus suggesting that RET tyrosine kinase plays a role in the neuronal response to axotomy and/or during nerve regeneration.
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UR - http://www.scopus.com/inward/citedby.url?scp=0039818903&partnerID=8YFLogxK
M3 - Article
C2 - 8853904
AN - SCOPUS:0039818903
VL - 7
SP - 1081
EP - 1086
JO - Cell Growth and Differentiation
JF - Cell Growth and Differentiation
SN - 1044-9523
IS - 8
ER -