Presence of serum IgD and IgD-containing plasma cells in the mouse

A. Bargellesi, G. Corte, E. Cosulich, M. Ferrarini

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A new technique which makes use of anti-δ-specific allo- or heteroantisera coupled to Sepharose 4B has been employed to investigate the presence of IgD molecules in the sera of three mouse strains. The sera were reacted with the insoluble immunosorbents, and the eluted material was labeled with 125I prior to antigenic or sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. This material reacted with anti-δ or anti-L chain reagents, but not with other anti-class-specific antisera and, after reduction, was resolved in three discrete bands in SDS-PAGE. The fastest of these bands comigrated with L chain, whereas the other two moved identically to mouse lymphocyte surface δ chains. Immunofluorescence studies on fixed smears of spleen and Peyer's patches' cells revealed the presence of a small but consistent number of IgD-containing plasma cells.

Original languageEnglish
Pages (from-to)490-492
Number of pages3
JournalEuropean Journal of Immunology
Volume9
Issue number6
Publication statusPublished - 1979

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Immunoglobulin D
Plasma Cells
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Peyer's Patches
Immunosorbents
Serum
Sepharose
Fluorescent Antibody Technique
Immune Sera
Spleen
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Bargellesi, A., Corte, G., Cosulich, E., & Ferrarini, M. (1979). Presence of serum IgD and IgD-containing plasma cells in the mouse. European Journal of Immunology, 9(6), 490-492.

Presence of serum IgD and IgD-containing plasma cells in the mouse. / Bargellesi, A.; Corte, G.; Cosulich, E.; Ferrarini, M.

In: European Journal of Immunology, Vol. 9, No. 6, 1979, p. 490-492.

Research output: Contribution to journalArticle

Bargellesi, A, Corte, G, Cosulich, E & Ferrarini, M 1979, 'Presence of serum IgD and IgD-containing plasma cells in the mouse', European Journal of Immunology, vol. 9, no. 6, pp. 490-492.
Bargellesi A, Corte G, Cosulich E, Ferrarini M. Presence of serum IgD and IgD-containing plasma cells in the mouse. European Journal of Immunology. 1979;9(6):490-492.
Bargellesi, A. ; Corte, G. ; Cosulich, E. ; Ferrarini, M. / Presence of serum IgD and IgD-containing plasma cells in the mouse. In: European Journal of Immunology. 1979 ; Vol. 9, No. 6. pp. 490-492.
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