Presenilin 2 is secreted in mouse primary neurons: A release enhanced by apoptosis

Roberta Ghidoni; Anna Paterlini; Luisa Benussi; Giuliano Binetti

doi:10.1016/j.mad.2007.01.003

Presenilin 2 is secreted in mouse primary neurons: A release enhanced by apoptosis

Roberta Ghidoni, Anna Paterlini, Luisa Benussi, Giuliano Binetti

Centro San Giovanni di Dio - Fatebenefratelli

Research output: Contribution to journal › Article › peer-review

Abstract

Presenilin 1 (PS1) and presenilin 2 (PS2) are homologous transmembrane proteins genetically associated with Alzheimer disease. As previously reported by our group for PS1, here we demonstrated that, in mouse primary neurons and microglial cells, PS2 C-terminal fragment (CTF) is released by shedding into the extracellular compartment as a soluble form and that this release is 4.07-fold increased during apoptosis. When compared with PS1, PS2 seems to be more susceptible to apoptosis since its secretion is increased 2.8-fold more than PS1. During apoptosis either proteins were colocalized especially within shedded vesicles. The present study suggest an active role for the presenilins CTF on putative target cells.

Original language	English
Pages (from-to)	350-353
Number of pages	4
Journal	Mechanisms of Ageing and Development
Volume	128
Issue number	4
DOIs	https://doi.org/10.1016/j.mad.2007.01.003
Publication status	Published - Apr 2007

Keywords

Alzheimer disease
Apoptosis
Confocal imaging
Mouse primary neurones
Presenilin
Shedding

ASJC Scopus subject areas

Ageing
Biochemistry
Developmental Biology
Developmental Neuroscience

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10.1016/j.mad.2007.01.003

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title = "Presenilin 2 is secreted in mouse primary neurons: A release enhanced by apoptosis",

abstract = "Presenilin 1 (PS1) and presenilin 2 (PS2) are homologous transmembrane proteins genetically associated with Alzheimer disease. As previously reported by our group for PS1, here we demonstrated that, in mouse primary neurons and microglial cells, PS2 C-terminal fragment (CTF) is released by shedding into the extracellular compartment as a soluble form and that this release is 4.07-fold increased during apoptosis. When compared with PS1, PS2 seems to be more susceptible to apoptosis since its secretion is increased 2.8-fold more than PS1. During apoptosis either proteins were colocalized especially within shedded vesicles. The present study suggest an active role for the presenilins CTF on putative target cells.",

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AU - Ghidoni, Roberta

AU - Paterlini, Anna

AU - Benussi, Luisa

AU - Binetti, Giuliano

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AB - Presenilin 1 (PS1) and presenilin 2 (PS2) are homologous transmembrane proteins genetically associated with Alzheimer disease. As previously reported by our group for PS1, here we demonstrated that, in mouse primary neurons and microglial cells, PS2 C-terminal fragment (CTF) is released by shedding into the extracellular compartment as a soluble form and that this release is 4.07-fold increased during apoptosis. When compared with PS1, PS2 seems to be more susceptible to apoptosis since its secretion is increased 2.8-fold more than PS1. During apoptosis either proteins were colocalized especially within shedded vesicles. The present study suggest an active role for the presenilins CTF on putative target cells.

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