Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy

Agnese Molinari, Graziella Orefici, Gianfranco Donelli, Cristina Von Hunolstein, Silvia Paradisi, Giuseppe Arancia

Research output: Contribution to journalArticle

Abstract

We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria, Streptococcus agalactiae and Streptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalHistochemical Journal
Volume20
Issue number9
DOIs
Publication statusPublished - Sep 1988

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ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Molinari, A., Orefici, G., Donelli, G., Von Hunolstein, C., Paradisi, S., & Arancia, G. (1988). Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy. Histochemical Journal, 20(9), 526-530. https://doi.org/10.1007/BF01002651