Pressure distension stimulates the expression of endothelial adhesion molecules in the human saphenous vein graft

Massimo Chello, Pasquale Mastroroberto, Giacomo Frati, Giuseppe Patti, Andrea D'Ambrosio, Germano Di Sciascio, Elvio Covino

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background. Mechanical trauma occurring during saphenous vein graft harvesting plays a major role in graft failure after coronary bypass surgery. There is increasing evidence that neutrophil-endothelial interaction is involved in the pathogenesis of early graft occlusion. This study evaluates the effect of pressure distension on the expression of endothelial adhesion molecules in human saphenous vein. Methods. Segments of saphenous vein graft (SVG) were collected from 20 patients undergoing coronary bypass surgery. We evaluated the expression of intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), and P-selectin on SVG endothelium under basal conditions and after pressure distension at 300 mm Hg. In the same experimental setting we also evaluated adhesion of both unstimulated and activated neutrophils to the endothelium of SVG. Results. Control endothelial cells exhibited only a weak staining for intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), and P-selectin, whereas the levels of adhesion molecules increased significantly in the distended veins. Similarly, significantly greater adhesion of both unstimulated and activated neutrophils was observed in distended veins compared with control veins. Conclusions. Pressure distension of SVG before coronary bypass surgery induces upregulation of endothelial adhesion molecules, with subsequent increase in neutrophil adhesion to the endothelium. Neutrophil adhesion to endothelial cells may contribute to early failure of SVG.

Original languageEnglish
Pages (from-to)453-458
Number of pages6
JournalAnnals of Thoracic Surgery
Volume76
Issue number2
DOIs
Publication statusPublished - Aug 1 2003

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Saphenous Vein
Transplants
Pressure
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Neutrophils
Endothelium
Veins
P-Selectin
Endothelial Cells
Up-Regulation
Staining and Labeling
Wounds and Injuries

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

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Pressure distension stimulates the expression of endothelial adhesion molecules in the human saphenous vein graft. / Chello, Massimo; Mastroroberto, Pasquale; Frati, Giacomo; Patti, Giuseppe; D'Ambrosio, Andrea; Di Sciascio, Germano; Covino, Elvio.

In: Annals of Thoracic Surgery, Vol. 76, No. 2, 01.08.2003, p. 453-458.

Research output: Contribution to journalArticle

Chello, Massimo ; Mastroroberto, Pasquale ; Frati, Giacomo ; Patti, Giuseppe ; D'Ambrosio, Andrea ; Di Sciascio, Germano ; Covino, Elvio. / Pressure distension stimulates the expression of endothelial adhesion molecules in the human saphenous vein graft. In: Annals of Thoracic Surgery. 2003 ; Vol. 76, No. 2. pp. 453-458.
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