Pressure-induced vascular oxidative stress is mediated through activation of integrin-linked kinase 1/βPIX/Rac-1 pathway

Carmine Vecchione, Daniela Carnevale, Alba Di Pardo, Maria Teresa Gentile, Antonio Damato, Germana Cocozza, Giovanna Antenucci, Giada Mascio, Umberto Bettarini, Alessandro Landolfi, Luca Iorio, Angelo Maffei, Giuseppe Lembo

Research output: Contribution to journalArticlepeer-review

Abstract

High blood pressure induces a mechanical stress on vascular walls and evokes oxidative stress and vascular dysfunction. The aim of this study was to characterize the intracellular signaling causing vascular oxidative stress in response to pressure. In carotid arteries subjected to high pressure levels, we observed not only an impaired vasorelaxation, increased superoxide production, and NADPH oxidase activity, but also a concomitant activation of Rac-1, a small G protein. Selective inhibition of Rac-1, with an adenovirus carrying a dominant-negative Rac-1 mutant, significantly reduced NADPH oxidase activity and oxidative stress and, more importantly, rescued vascular function in carotid arteries at high pressure. The analysis of molecular events associated with mechanotransduction demonstrated at high pressure levels an overexpression of integrin-linked kinase 1 and its recruitment to plasma membrane interacting with paxillin. The inhibition of integrin-linked kinase 1 by small interfering RNA impaired Rac-1 activation and rescued oxidative stress-induced vascular dysfunction in response to high pressure. Finally, we showed that βPIX, a guanine-nucleotide exchange factor, is the intermediate molecule recruited by integrin-linked kinase 1, converging the intracellular signaling toward Rac-1-mediated oxidative vascular dysfunction during pressure overload. Our data demonstrate that biomechanical stress evoked by high blood pressure triggers an integrin-linked kinase 1/βPIX/Rac-1 signaling, thus generating oxidative vascular dysfunction.

Original languageEnglish
Pages (from-to)1028-1034
Number of pages7
JournalHypertension
Volume54
Issue number5
DOIs
Publication statusPublished - Nov 2009

Keywords

  • Endothelial dysfunction
  • High pressure
  • Integrin signaling
  • Mechanotransduction
  • Oxidative stress

ASJC Scopus subject areas

  • Internal Medicine

Fingerprint Dive into the research topics of 'Pressure-induced vascular oxidative stress is mediated through activation of integrin-linked kinase 1/βPIX/Rac-1 pathway'. Together they form a unique fingerprint.

Cite this