Presynaptic component of long-term potentiation visualized at individual hippocampal synapses

Antonio Malgaroli, Anthony E. Ting, Beverly Wendland, Andrea Bergamaschi, Antonello Villa, Richard W. Tsien, Richard H. Scheller

Research output: Contribution to journalArticlepeer-review

Abstract

Long-term potentiation has previously been studied with electrophysiological techniques that do not readily separate presynaptic and postsynaptic contributions. Changes in exocytotic-endocytotic cycling have now been monitored at synapses between cultured rat hippocampal neurons by measuring the differential uptake of antibodies that recognize the intraluminal domain of the synaptic vesicle protein synaptotagmin. Vesicular cycling increased markedly during glutamate-induced long-term potentiation. The degree of potentiation was heterogeneous, appearing greater at synapses at which the initial extent of vesicular turnover was low. Thus, changes in presynaptic activity were visualized directly and the spatial distribution of potentiation could be determined at the level of single synaptic boutons.

Original languageEnglish
Pages (from-to)1624-1628
Number of pages5
JournalScience
Volume268
Issue number5217
Publication statusPublished - 1995

ASJC Scopus subject areas

  • General

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