Prevailingly cationic agmatine-based amphoteric polyamidoamine as a nontoxic, nonhemolytic, and "stealthlike" DNA complexing agent and transfection promoter

Paolo Ferruti, Jacopo Franchini, Marco Bencini, Elisabetta Ranucci, Gian Paolo Zara, Loredana Serpe, Luca Primo, Roberta Cavalli

Research output: Contribution to journalArticle

Abstract

AGMA1, a prevailingly cationic amphoteric polyamidoamine obtained by polyaddition of (4-aminobutyl)guanidine (agmatine) to 2,2-bis(acrylamido)acetic acid, was studied as a potential DNA carrier and transfection promoter. Fluorescein-labeled AGMA1 was prepared by conjugation with fluorescein isothiocyanate and its cell uptake, blood permanence, and body distribution studied. In spite of its cationic character, AGMA1 is neither toxic nor hemolytic in the pH range 4.0-7.4, circulates for a long time in the blood without preferentially localizing in the liver, easily enters HT-29 cells, gives stable complexes with DNA, and is endowed with good transfection efficiency, suggesting the ability to transport in the cytoplasm a DNA payload without any measurable membranolytic activity. If compared with other transfection promoters, including polyamidoamines of different structures, AGMA1 is apparently endowed with a unique combination of desirable requirements for a nonviral DNA polymer carrier and warrants potential as a transfection agent in vivo.

Original languageEnglish
Pages (from-to)1498-1504
Number of pages7
JournalBiomacromolecules
Volume8
Issue number5
DOIs
Publication statusPublished - May 2007

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Agmatine
Transfection
DNA
Fluorescein
Blood
HT29 Cells
Poisons
Acetic acid
Acetic Acid
Liver
Blood Cells
Polymers
Cytoplasm
Poly(amidoamine)

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Prevailingly cationic agmatine-based amphoteric polyamidoamine as a nontoxic, nonhemolytic, and "stealthlike" DNA complexing agent and transfection promoter. / Ferruti, Paolo; Franchini, Jacopo; Bencini, Marco; Ranucci, Elisabetta; Zara, Gian Paolo; Serpe, Loredana; Primo, Luca; Cavalli, Roberta.

In: Biomacromolecules, Vol. 8, No. 5, 05.2007, p. 1498-1504.

Research output: Contribution to journalArticle

Ferruti, Paolo ; Franchini, Jacopo ; Bencini, Marco ; Ranucci, Elisabetta ; Zara, Gian Paolo ; Serpe, Loredana ; Primo, Luca ; Cavalli, Roberta. / Prevailingly cationic agmatine-based amphoteric polyamidoamine as a nontoxic, nonhemolytic, and "stealthlike" DNA complexing agent and transfection promoter. In: Biomacromolecules. 2007 ; Vol. 8, No. 5. pp. 1498-1504.
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