Prevalence and biological effects of anti-trophoblast and anti-endothelial cell antibodies in patients with recurrent spontaneous abortions

F. Tedesco, M. Pausa, E. Nardon, G. Narchi, R. Bulla, C. Livi, S. Guaschino, P. L. Meroni

Research output: Contribution to journalArticlepeer-review

Abstract

Problem: Trophoblasts and endothelial cells represent a potential target for antibodies in women with recurrent spontaneous abortions. These antibodies have been shown to be associated with anti-phospholipid antibodies. Are they also present in women with unexplained pregnancy losses in the absence of anti-phospholipid antibodies? Method of Study: The anti-trophoblast antibodies were tested by an immunofluorescence assay on cells purified from pooled first-trimester placentae, whereas the anti-endothelial cell antibodies were measured by enzyme-linked immunoadsorbent assay (ELISA) on cells isolated from the umbilical vein and were cultured to confluence. The cytotoxicity of trophoblasts was evaluated in a homologous system. The expression of adhesion molecules on endothelial cells was quantitated by ELISA using specific monoclonal antibodies, and the expression of tissue factor was quantitated by a chromogenic assay measuring the formation of factor Xa. Results and Conclusions: Complement-fixing antibodies to trophoblast represent a better marker to discriminate patients with recurrent spontaneous abortions from controls and are cytotoxic for the target cells. Anti-endothelial antibodies are also present in these patients and exhibit pro-inflammatory and pro-coagulant activities.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume38
Issue number3
Publication statusPublished - 1997

Keywords

  • Abortions
  • Adhesion molecules
  • Antibodies
  • Complement
  • Endothelial cells
  • Trophoblast

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

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